Signal Transduction Antibodies 2
Anti-UBA5 Antibody (CAB15514)
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- SKU:
- CAB15514
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UBA5 Antibody |
| Antibody SKU: | CAB15514 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UBA5 (NP_079094.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, A-549, Jurkat, HeLa, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UBA5 (NP_079094.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAES VERL QQRV QELE RELA QERS LQVP RSGD GGGG RVRI EKMS SEVV DSNP YSRL MALK RMGI VSDY EKIR TFAV AIVG VGGV GSVT AEML TRCG IGKL LLFD YDKV ELAN MNRL FFQP HQAG LSKV QAAE HTLR NINP DVLF EVHN YNIT TVEN FQHF MDRI SNGG LEEG KPVD LVLS CVDN FEAR MTIN TACN ELGQ TWME SGVS ENAV SGHI QLII PGES ACFA CAPP LVVA ANID EKTL KREG VCAA SLPT TMGV VAGI LVQN VLKF LLNF GTVS FYLG YNAM QDFF PTMS MKPN PQCD DRNC RKQQ EEYK KKVA ALPK QEVI QEEE EIIH EDNE WGIE LVSE VSEE ELKN FSGP VPDL PEGI TVAY TIPK KQED SVTE LTVE DSGE SLED LMAK MKNM |
| Gene ID: | 79876 |
| Uniprot: | Q9GZZ9 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 38kDa/44kDa |
| Observed MW: | 50kDa |
| Synonyms: | UBA5, EIEE44, SCAR24, THIFP1, UBE1DC1 |
| Background: | This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. |
| UniProt Protein Function: | E1-like enzyme which activates UFM1 and SUMO2. |
| NCBI Summary: | This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. [provided by RefSeq, Feb 2016] |
| UniProt Code: | Q9GZZ9 |
| NCBI GenInfo Identifier: | 13376212 |
| NCBI Gene ID: | 79876 |
| NCBI Accession: | NP_079094 |
| UniProt Secondary Accession: | Q9GZZ9,Q96ST1, A6NJL3, D3DNC8, |
| UniProt Related Accession: | Q9GZZ9 |
| Molecular Weight: | 47.4kDa (428aa) confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin-like modifier-activating enzyme 5 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin like modifier activating enzyme 5 |
| NCBI Official Symbol: | UBA5 |
| NCBI Official Synonym Symbols: | EIEE44; SCAR24; THIFP1; UBE1DC1 |
| NCBI Protein Information: | ubiquitin-like modifier-activating enzyme 5 |
| UniProt Protein Name: | Ubiquitin-like modifier-activating enzyme 5 |
| UniProt Synonym Protein Names: | ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1 |
| UniProt Gene Name: | UBA5 |
 | Western blot analysis of extracts of various cell lines, using UBA5 antibody (CAB15514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded Rat liver using UBA5 Rabbit pAb (CAB15514) at dilution of 1:100 (40x lens). |
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