Cell Cycle Antibodies 1
Anti-UBA3 Antibody (CAB15119)
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- SKU:
- CAB15119
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-UBA3 Antibody |
| Antibody SKU: | CAB15119 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-463 of human UBA3 (NP_003959.3). |
| Application: | IF |
| Recommended Dilution: | IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-463 of human UBA3 (NP_003959.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FPMC TIAS MPRL PEHC IEYV RMLQ WPKE QPFG EGVP LDGD DPEH IQWI FQKS LERA SQYN IRGV TYRL TQGV VKRI IPAV ASTN AVIA AVCA TEVF KIAT SAYI PLNN YLVF NDVD GLYT YTFE AERK ENCP ACSQ LPQN IQFS PSAK LQEV LDYL TNSA SLQM KSPA ITAT LEGK NRTL YLQS VTSI EERT RPNL SKTL KELG LVDG QELA VADV TTPQ TVLF KLHF TS |
| Gene ID: | 9039 |
| Uniprot: | Q8TBC4 |
| Cellular Location: | |
| Calculated MW: | 50kDa/51kDa |
| Observed MW: |
| Synonyms: | UBA3, NAE2, UBE1C, hUBA3 |
| Background: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; Cell cycle regulation; EC 6.3.2.- Chromosomal Location of Human Ortholog: 3p14.1 Cellular Component: nucleus; cytosol Molecular Function:NEDD8 activating enzyme activity; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; ATP binding Biological Process: protein neddylation; regulation of cell cycle; protein modification process; endomitotic cell cycle; proteolysis |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8TBC4 |
| NCBI GenInfo Identifier: | 83305811 |
| NCBI Gene ID: | 9039 |
| NCBI Accession: | Q8TBC4.2 |
| UniProt Related Accession: | Q8TBC4 |
| Molecular Weight: | |
| NCBI Full Name: | NEDD8-activating enzyme E1 catalytic subunit |
| NCBI Synonym Full Names: | ubiquitin like modifier activating enzyme 3 |
| NCBI Official Symbol: | UBA3 |
| NCBI Official Synonym Symbols: | NAE2; UBE1C; hUBA3 |
| NCBI Protein Information: | NEDD8-activating enzyme E1 catalytic subunit |
| UniProt Protein Name: | NEDD8-activating enzyme E1 catalytic subunit |
| UniProt Synonym Protein Names: | NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3 |
| Protein Family: | Ubiquitin-activating enzyme |
| UniProt Gene Name: | UBA3 |
| UniProt Entry Name: | UBA3_HUMAN |
 | Immunofluorescence analysis of U2OS cells using UBA3 antibody (CAB15119) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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