Signal Transduction Antibodies 2

Anti-UBA2 Antibody (CAB17342)

(No reviews yet) Write a Review
SKU:
CAB17342
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-UBA2 Antibody
Antibody SKU:CAB17342
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-160 of human UBA2 (NP_005490.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Rat
Positive Samples:Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-160 of human UBA2 (NP_005490.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Isotype:IgG
Sequence:QFLF QKKH VGRS KAQV AKES VLQF YPKA NIVA YHDS IMNP DYNV EFFR QFIL VMNA LDNR AARN HVNR MCLA ADVP LIES GTAG YLGQ VTTI KKGV TECY E
Gene ID:10054
Uniprot:Q9UBT2
Cellular Location:Cytoplasm, Nucleus
Calculated MW:60kDa/71kDa
Observed MW:90kDa
Synonyms:UBA2, ARX, HRIHFB2115, SAE2
Background:
UniProt Protein Function:SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Motility/polarity/chemotaxis; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function:SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification

NCBI Summary:Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
UniProt Code:Q9UBT2
NCBI GenInfo Identifier:42559898
NCBI Gene ID:10054
NCBI Accession:Q9UBT2.2
UniProt Secondary Accession:Q9UBT2,O95605, Q59H87, Q6IBP6, Q9NTJ1, Q9UED2, B3KWB9
UniProt Related Accession:Q9UBT2
Molecular Weight:640
NCBI Full Name:SUMO-activating enzyme subunit 2
NCBI Synonym Full Names:ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol:UBA2  
NCBI Official Synonym Symbols:ARX; SAE2; HRIHFB2115  
NCBI Protein Information:SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; ubiquitin-like modifier-activating enzyme 2; UBA2, ubiquitin-activating enzyme E1 homolog
UniProt Protein Name:SUMO-activating enzyme subunit 2
UniProt Synonym Protein Names:Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2
Protein Family:Ubiquitin-activating enzyme
UniProt Gene Name:UBA2  
UniProt Entry Name:SAE2_HUMAN
Anti-UBA2 Antibody (CAB17342)Western blot analysis of extracts of Rat lung, using UBA2 antibody (CAB17342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
View AllClose

0 Reviews

View AllClose

Related Products