Signal Transduction Antibodies 1
Anti-UBA1 Antibody (CAB12359)
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- SKU:
- CAB12359
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UBA1 Antibody |
| Antibody SKU: | CAB12359 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KISF KSLV ASLA EPDF VVTD FAKF SRPA QLHI GFQA LHQF CAQH GRPP RPRN EEDA AELV ALAQ AVNA RALP AVQQ NNLD EDLI RKLA YVAA GDLA PINA FIGG LAAQ EVMK ACSG KFMP IMQW LYFD ALEC LPED KEVL TEDK CLQR QNRY DGQV AVFG SDLQ EKLG KQKY FLVG AGAI GCEL LKNF AMIG LGCG EGGE IIVT DMDT IEKS NLNR QFLF RPWD VTKL KSDT AAAA VRQM NPHI RVTS HQNR VGPD TERI YDDD FFQN LDGV ANAL DNVD ARMY MDRR CVYY RKPL LESG T |
| Gene ID: | 7317 |
| Uniprot: | P22314 |
| Cellular Location: | Cytoplasm, Mitochondrion, Nucleus, Nucleus |
| Calculated MW: | 113kDa/117kDa |
| Observed MW: | 121kDa |
| Synonyms: | UBA1, A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X |
| Background: | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. |
| UniProt Protein Function: | UBE1: an enzyme of the ubiquitin-activating E1 family. Adenylates ubiquitin at its carboxy-terminal glycine residue and thereafter links this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester. There are two active sites within the E1 molecule, allowing it to accomodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: heterochromatin; desmosome; mitochondrion; lysosomal membrane; cytoplasm; endosome membrane; rough endoplasmic reticulum membrane; nucleus; cytosol Molecular Function:protein binding; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding Biological Process: modification-dependent protein catabolic process; protein ubiquitination; response to DNA damage stimulus Disease: Spinal Muscular Atrophy, X-linked 2 |
| NCBI Summary: | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22314 |
| NCBI GenInfo Identifier: | 24418865 |
| NCBI Gene ID: | 7317 |
| NCBI Accession: | P22314.3 |
| UniProt Secondary Accession: | P22314,Q5JRR8, Q96E13, |
| UniProt Related Accession: | P22314 |
| Molecular Weight: | 1058 |
| NCBI Full Name: | Ubiquitin-like modifier-activating enzyme 1 |
| NCBI Synonym Full Names: | ubiquitin-like modifier activating enzyme 1 |
| NCBI Official Symbol: | UBA1 |
| NCBI Official Synonym Symbols: | A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124 |
| NCBI Protein Information: | ubiquitin-like modifier-activating enzyme 1; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing |
| UniProt Protein Name: | Ubiquitin-like modifier-activating enzyme 1 |
| UniProt Synonym Protein Names: | Protein A1S9; Ubiquitin-activating enzyme E1 |
| UniProt Gene Name: | UBA1 |
| UniProt Entry Name: | UBA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBA1 antibody (CAB12359) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Rat liver using UBA1 antibody (CAB12359) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using UBA1 antibody (CAB12359) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse brain using UBA1 antibody (CAB12359) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using UBA1 Rabbit pAb (CAB12359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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