Antibody Name:	Anti-U2AF1 Antibody
Antibody SKU:	CAB6076
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	Raji, SW620

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEY LASI FGTE KDKV NCSF YFKI GACR HGDR CSRL HNKP TFSQ TIAL LNIY RNPQ NSSQ SADG LRCA VSDV EMQE HYDE FFEE VFTE MEEK YGEV EEMN VCDN LGDH LVGN VYVK FRRE EDAE KAVI DLNN RWFN GQPI HAEL SPVT DFRE ACCR QYEM GECT RGGF CNFM HLKP ISRE LRRE LYGR RRKK HRSR SRSR ERRS RSRD RGRG GGGG GGGG GGGR ERDR RRSR DRER SGRF
Gene ID:	7307
Uniprot:	Q01081
Cellular Location:	Nucleus, Nucleus speckle
Calculated MW:	8kDa/19kDa/27kDa
Observed MW:	38kDa

Synonyms:

U2AF1, FP793, RN, RNU2AF1, U2AF35, U2AFBP

Background:

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; RNA splicing; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck Molecular Function:RS domain binding; protein binding; metal ion binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
NCBI Summary:	This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01081
NCBI GenInfo Identifier:	267187
NCBI Gene ID:	7307
NCBI Accession:	Q01081.3
UniProt Secondary Accession:	Q01081,Q701P4, Q71RF1,
UniProt Related Accession:	Q01081
Molecular Weight:	240
NCBI Full Name:	Splicing factor U2AF 35 kDa subunit
NCBI Synonym Full Names:	U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol:	U2AF1
NCBI Official Synonym Symbols:	RN; FP793; U2AF35; U2AFBP; RNU2AF1
NCBI Protein Information:	splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit
UniProt Protein Name:	Splicing factor U2AF 35 kDa subunit
UniProt Synonym Protein Names:	U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit
Protein Family:	Splicing factor
UniProt Gene Name:	U2AF1
UniProt Entry Name:	U2AF1_HUMAN

	Western blot analysis of extracts of various cell lines, using U2AF1 antibody (CAB6076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A-549 cells using U2AF1 antibody (CAB6076). Blue: DAPI for nuclear staining.