Antibody Name:	Anti-U2AF1 Antibody
Antibody SKU:	CAB13166
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, K-562, LO2, Mouse spleen, Mouse kidney, Rat spleen

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U2AF1 (NP_006749.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEY LASI FGTE KDKV NCSF YFKI GACR HGDR CSRL HNKP TFSQ TIAL LNIY RNPQ NSSQ SADG LRCA VSDV EMQE HYDE FFEE VFTE MEEK YGEV EEMN
Gene ID:	7307
Uniprot:	Q01081
Cellular Location:	Nucleus, Nucleus speckle
Calculated MW:	8kDa/19kDa/27kDa
Observed MW:	38kDa

Synonyms:

U2AF1, FP793, RN, RNU2AF1, U2AF35, U2AFBP

Background:

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; RNA splicing; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck Molecular Function:RS domain binding; protein binding; metal ion binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
NCBI Summary:	This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01081
NCBI GenInfo Identifier:	267187
NCBI Gene ID:	7307
NCBI Accession:	Q01081.3
UniProt Secondary Accession:	Q01081,Q701P4, Q71RF1,
UniProt Related Accession:	Q01081
Molecular Weight:	240
NCBI Full Name:	Splicing factor U2AF 35 kDa subunit
NCBI Synonym Full Names:	U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol:	U2AF1
NCBI Official Synonym Symbols:	RN; FP793; U2AF35; U2AFBP; RNU2AF1
NCBI Protein Information:	splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit
UniProt Protein Name:	Splicing factor U2AF 35 kDa subunit
UniProt Synonym Protein Names:	U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit
Protein Family:	Splicing factor
UniProt Gene Name:	U2AF1
UniProt Entry Name:	U2AF1_HUMAN

	Western blot analysis of extracts of various cell lines, using U2AF1 antibody (CAB13166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human lung cancer using U2AF1 antibody (CAB13166) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug U2AF1 antibody (CAB13166). Western blot was performed from the immunoprecipitate using U2AF1 antibody (CAB13166) at a dilition of 1:1000.