Antibody Name:	Anti-TYMS Antibody
Antibody SKU:	CAB10441
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human TYMS (NP_001062.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, Jurkat, C6, Mouse liver, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human TYMS (NP_001062.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPVA GSEL PRRP LPPA AQER DAEP RPPH GELQ YLGQ IQHI LRCG VRKD DRTG TGTL SVFG MQAR YSLR DEFP LLTT KRVF WKGV LEEL LWFI KGST NAKE LSSK GVKI WDAN GSRD FLDS LGFS TREE GDLG PVYG FQWR HFGA EYRD MESD YSGQ GVDQ LQRV IDTI KTNP DDRR IIMC AWNP RDLP LMAL PPCH ALCQ FYVV NSEL SCQL YQRS GDMG LGVP FNIA SYAL LTYM IAHI TGLK PGDF IHTL GDAH IYLN HIEP LKIQ LQRE PRPF PKLR ILRK VEKI DDFK AEDF QIEG YNPH PTIK MEMA V
Gene ID:	7298
Uniprot:	P04818
Cellular Location:	Cytoplasm, Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, Nucleus
Calculated MW:	26kDa/31kDa/35kDa
Observed MW:	36KDa

Synonyms:

TYMS, HST422, TMS, TS

Background:

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.

UniProt Protein Function:	TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Mitochondrial; Methyltransferase Chromosomal Location of Human Ortholog: 18p11.32 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol Molecular Function:mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding Biological Process: immortalization of host cell by virus; methylation; tetrahydrofolate metabolic process; circadian rhythm; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle
NCBI Summary:	Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]
UniProt Code:	P04818
NCBI GenInfo Identifier:	136611
NCBI Gene ID:	7298
NCBI Accession:	P04818.3
UniProt Secondary Accession:	P04818,Q8WYK3, Q8WYK4,
UniProt Related Accession:	P04818
Molecular Weight:	Calculated MW: 26kDa/31kDa/35kDaObserved MW: 36kDa
NCBI Full Name:	Thymidylate synthase
NCBI Synonym Full Names:	thymidylate synthetase
NCBI Official Symbol:	TYMS
NCBI Official Synonym Symbols:	TS; TMS; HST422
NCBI Protein Information:	thymidylate synthase; TSase
UniProt Protein Name:	Thymidylate synthase
Protein Family:	Thymidylate synthase
UniProt Gene Name:	TYMS
UniProt Entry Name:	TYSY_HUMAN

	Western blot analysis of extracts of various cell lines, using TYMS antibody (CAB10441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00026). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using TYMS antibody (CAB10441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse spleen using TYMS Rabbit pAb (CAB10441) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using TYMS Rabbit pAb (CAB10441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using TYMS Rabbit pAb (CAB10441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.