Antibody Name:	Anti-TYMP Antibody
Antibody SKU:	CAB1094
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TYMP (NP_001107228.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	A-549, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TYMP (NP_001107228.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL MTPG TGAP PAPG DFSG EGSQ GLPD PSPE PKQL PELI RMKR DGGR LSEA DIRG FVAA VVNG SAQG AQIG AMLM AIRL RGMD LEET SVLT QALA QSGQ QLEW PEAW RQQL VDKH STGG VGDK VSLV LAPA LAAC GCKV PMIS GRGL GHTG GTLD KLES IPGF NVIQ SPEQ MQVL LDQA GCCI VGQS EQLV PADG ILYA
Gene ID:	1890
Uniprot:	P19971
Cellular Location:
Calculated MW:	49kDa/50kDa
Observed MW:	50kDa

Synonyms:

TYMP, ECGF, ECGF1, MEDPS1, MNGIE, MTDPS1, PDECGF, TP, hPD-ECGF

Background:

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified.

UniProt Protein Function:	ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Nucleotide Metabolism - pyrimidine; EC 2.4.2.4; Phosphorylase; DNA replication; Transferase; Cytokine; Xenobiotic Metabolism - drug metabolism - other enzymes Chromosomal Location of Human Ortholog: 22q13.33 Cellular Component: cytosol Molecular Function:phosphorylase activity; platelet-derived growth factor receptor binding; transferase activity, transferring pentosyl groups Biological Process: DNA replication; mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine nucleoside salvage; pyrimidine nucleotide metabolic process Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
NCBI Summary:	This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Code:	P19971
NCBI GenInfo Identifier:	67477361
NCBI Gene ID:	1890
NCBI Accession:	P19971.2
UniProt Secondary Accession:	P19971,Q13390, Q8WVB7, A8MW15, H9KVA0,
UniProt Related Accession:	P19971
Molecular Weight:	50,405 Da
NCBI Full Name:	Thymidine phosphorylase
NCBI Synonym Full Names:	thymidine phosphorylase
NCBI Official Symbol:	TYMP
NCBI Official Synonym Symbols:	TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
NCBI Protein Information:	thymidine phosphorylase
UniProt Protein Name:	Thymidine phosphorylase
UniProt Synonym Protein Names:	Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
UniProt Gene Name:	TYMP
UniProt Entry Name:	TYPH_HUMAN

	Western blot analysis of extracts of various cell lines, using TYMP antibody (CAB1094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using TYMP antibody (CAB1094). Blue: DAPI for nuclear staining.