Cell Biology Antibodies 9

Anti-TXNRD2 Antibody (CAB5490)

(No reviews yet) Write a Review
SKU:
CAB5490
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TXNRD2 Antibody
Antibody SKU:CAB5490
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T, HepG2, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AALL GGLI QDAP NYGW EVAQ PVPH DWRK MAEA VQNH VKSL NWGH RVQL QDRK VKYF NIKA SFVD EHTV CGVA KGGK EILL SADH IIIA TGGR PRYP THIE GALE YGIT SDDI FWLK ESPG KTLV VGAS YVAL ECAG FLTG IGLD TTIM MRSI PLRG FDQQ MSSM VIEH MASH GTRF LRGC APSR VRRL PDGQ LQVT WEDS TTGK EDTG TFD
Gene ID:10587
Uniprot:Q9NNW7
Cellular Location:Mitochondrion
Calculated MW:30kDa/46kDa/53kDa/56kDa
Observed MW:56kDa
Synonyms:TXNRD2, SELZ, TR, TR-BETA, TR3, TRXR2
Background:The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene.
UniProt Protein Function:TXNRD2: Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox- regulated cell signaling. Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; EC 1.8.1.9; Oxidoreductase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function:FAD binding; protein binding; thioredoxin-disulfide reductase activity

Biological Process: cell redox homeostasis; response to oxygen radical; response to reactive oxygen species

NCBI Summary:This gene encodes a member of the class I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein is a selenocysteine-containing flavoenzyme that maintains thioredoxins in a reduced state, thereby playing a key role in regulating the cellular redox environment. Mammals have three related thioredoxin reductases. This gene encodes a mitochondrial form important for scavenging of reactive oxygen species in mitochondria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2013]
UniProt Code:Q9NNW7
NCBI GenInfo Identifier:182705230
NCBI Gene ID:10587
NCBI Accession:Q9NNW7.3
UniProt Secondary Accession:Q9NNW7,O95840, Q96IJ2, Q9H2Z5, Q9NZV3, Q9NZV4, Q9P2Y0 Q9P2Y1, Q9UQU8,
UniProt Related Accession:Q9NNW7
Molecular Weight:30,291 Da
NCBI Full Name:Thioredoxin reductase 2, mitochondrial
NCBI Synonym Full Names:thioredoxin reductase 2
NCBI Official Symbol:TXNRD2  
NCBI Official Synonym Symbols:TR; TR3; SELZ; TRXR2; TR-BETA  
NCBI Protein Information:thioredoxin reductase 2, mitochondrial
UniProt Protein Name:Thioredoxin reductase 2, mitochondrial
UniProt Synonym Protein Names:Selenoprotein Z; SelZ; TR-beta; Thioredoxin reductase TR3
Protein Family:Thioredoxin reductase
UniProt Gene Name:TXNRD2  
UniProt Entry Name:TRXR2_HUMAN
Anti-TXNRD2 Antibody (CAB5490)Western blot analysis of extracts of various cell lines, using TXNRD2 antibody (CAB5490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TXNRD2 Antibody (CAB5490)Immunohistochemistry of paraffin-embedded human liver damage using TXNRD2 antibody (CAB5490) at dilution of 1:100 (40x lens).
Anti-TXNRD2 Antibody (CAB5490)Immunohistochemistry of paraffin-embedded human liver cancer using TXNRD2 antibody (CAB5490) at dilution of 1:100 (40x lens).
View AllClose

0 Reviews

View AllClose

Related Products