Signal Transduction Antibodies 2

Anti-TWF1 Antibody (CAB15307)

(No reviews yet) Write a Review
SKU:
CAB15307
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TWF1 Antibody
Antibody SKU:CAB15307
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human TWF1 (NP_002813.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-29, OVCAR3, DU145, Mouse brain, Mouse liver, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 101-350 of human TWF1 (NP_002813.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YAAT RATL KKEF GGGH IKDE VFGT VKED VSLH GYKK YLLS QSSP APLT AAEE ELRQ IKIN EVQT DVGV DTKH QTLQ GVAF PISR EAFQ ALEK LNNR QLNY VQLE IDIK NEII ILAN TTNT ELKD LPKR IPKD SARY HFFL YKHS HEGD YLES IVFI YSMP GYTC SIRE RMLY SSCK SRLL EIVE RQLQ MDVI RKIE IDNG DELT ADFL YEEV HPKQ HAHK QSFA KPKG PAGK RGIR RLIR GPAE TEAT TD
Gene ID:5756
Uniprot:Q12792
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:28kDa/40kDa/41kDa
Observed MW:40kDa
Synonyms:TWF1, A6, PTK9
Background:This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
UniProt Protein Function:A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: actin cytoskeleton; cytoplasm; filopodium; focal adhesion; intercellular junction; myofibril; perinuclear region of cytoplasm

Molecular Function:actin monomer binding; ATP binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity

Biological Process: negative regulation of actin filament polymerization; regulation of actin phosphorylation; sequestering of actin monomers

NCBI Summary:This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]
UniProt Code:Q12792
NCBI GenInfo Identifier:259016376
NCBI Gene ID:5756
NCBI Accession:Q12792.3
UniProt Secondary Accession:Q12792,Q59G07, Q5U0B1, Q6FHJ1, Q6FHL6, Q6NUK9, Q86XL6 Q8TCD3, A8K5A8, B3KXS6, B4DLX9,
UniProt Related Accession:Q12792
Molecular Weight:28,823 Da
NCBI Full Name:Twinfilin-1
NCBI Synonym Full Names:twinfilin actin binding protein 1
NCBI Official Symbol:TWF1  
NCBI Official Synonym Symbols:A6; PTK9  
NCBI Protein Information:twinfilin-1
UniProt Protein Name:Twinfilin-1
UniProt Synonym Protein Names:Protein A6; Protein tyrosine kinase 9
Protein Family:Twinfilin
UniProt Gene Name:TWF1  
UniProt Entry Name:TWF1_HUMAN
Anti-TWF1 Antibody (CAB15307)Western blot analysis of extracts of various cell lines, using TWF1 antibody (CAB15307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products