Immunology Antibodies 2
Anti-TUFM Antibody (CAB6423)
- SKU:
- CAB6423
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TUFM Antibody |
| Antibody SKU: | CAB6423 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, HepG2, HeLa, A-431, Jurkat, Mouse kidney, Mouse liver, Rat kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ADAV QDSE MVEL VELE IREL LTEF GYKG EETP VIVG SALC ALEG RDPE LGLK SVQK LLDA VDTY IPVP ARDL EKPF LLPV EAVY SVPG RGTV VTGT LERG ILKK GDEC ELLG HSKN IRTV VTGI EMFH KSLE RAEA GDNL GALV RGLK REDL RRGL VMVK PGSI KPHQ KVEA QVYI LSKE EGGR HKPF VSHF MPVM FSLT WDMA CRII LPPE KELA MPGE DLKF NLIL RQPM ILEK GQRF TLRD GNRT IGTG LVTN TLAM TEEE KNIK WG |
| Gene ID: | 7284 |
| Uniprot: | P49411 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 49kDa |
| Observed MW: | 48kDa |
| Synonyms: | TUFM, COXPD4, EF-TuMT, EFTU, P43 |
| Background: | This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. |
| UniProt Protein Function: | EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. |
| UniProt Protein Details: | Protein type:Translation elongation; RNA-binding; Translation; Mitochondrial Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: membrane; mitochondrion Molecular Function:GTP binding; GTPase activity; protein binding; translation elongation factor activity Biological Process: mitochondrial translation; organelle organization and biogenesis; translational elongation Disease: Combined Oxidative Phosphorylation Deficiency 4 |
| NCBI Summary: | This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49411 |
| NCBI GenInfo Identifier: | 1706611 |
| NCBI Gene ID: | 7284 |
| NCBI Accession: | P49411.2 |
| UniProt Secondary Accession: | P49411,O15276, |
| UniProt Related Accession: | P49411 |
| Molecular Weight: | |
| NCBI Full Name: | Elongation factor Tu, mitochondrial |
| NCBI Synonym Full Names: | Tu translation elongation factor, mitochondrial |
| NCBI Official Symbol: | TUFM |
| NCBI Official Synonym Symbols: | P43; EFTU; COXPD4; EF-TuMT |
| NCBI Protein Information: | elongation factor Tu, mitochondrial |
| UniProt Protein Name: | Elongation factor Tu, mitochondrial |
| UniProt Synonym Protein Names: | P43 |
| UniProt Gene Name: | TUFM |
| UniProt Entry Name: | EFTU_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TUFM antibody (CAB6423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody (CAB6423) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using TUFM antibody (CAB6423). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody (CAB6423). Western blot was performed from the immunoprecipitate using TUFM antibody (CAB6423) at a dilition of 1:1000. |
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