Cell Biology Antibodies 1

Anti-TUBGCP2 Antibody (CAB10283)

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SKU:
CAB10283
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TUBGCP2 Antibody
Antibody SKU:CAB10283
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TUBGCP2 (NP_006650.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TUBGCP2 (NP_006650.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSEF RIHH DVNE LLSL LRVH GGDG AEVY IDLL QKNR TPYV TTTV SAHS AKVK IAEF SRTP EDFL KKYD ELKS KNTR NLDP LVYL LSKL TEDK ETLQ YLQQ NAKE RAEL AAAA VGSS TTSI NVPA AASK ISMQ ELEE LRKQ LGSV ATGS TLQQ SLEL KRKM LRDK QNKK NSGQ HLPI FPAW
Gene ID:10844
Uniprot:Q9BSJ2
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:88kDa/102kDa/105kDa
Observed MW:102kDa
Synonyms:TUBGCP2, GCP-2, GCP2, Grip103, SPBC97, Spc97p, h103p, hGCP2, hSpc97
Background:
UniProt Protein Function:GCP2: the gamma-tubulin complex, composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6, is necessary for microtubule nucleation at the centrosome.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: centrosome; cytoplasmic microtubule; cytosol; gamma-tubulin small complex; membrane; microtubule organizing center; nucleoplasm; spindle pole body

Molecular Function:gamma-tubulin binding; microtubule minus-end binding; protein binding; structural constituent of cytoskeleton

Biological Process: centrosome duplication; cytoplasmic microtubule organization and biogenesis; interphase microtubule nucleation by interphase microtubule organizing center; microtubule nucleation; protein complex assembly

UniProt Code:Q9BSJ2
NCBI GenInfo Identifier:21450889
NCBI Gene ID:10844
NCBI Accession:Q9BSJ2.2
UniProt Secondary Accession:Q9BSJ2,O43632, Q5VWX7, B4DM18, B7ZKL8, F5H4E0, F5H4L0
UniProt Related Accession:Q9BSJ2
Molecular Weight:103kDa
NCBI Full Name:Gamma-tubulin complex component 2
NCBI Synonym Full Names:tubulin gamma complex associated protein 2
NCBI Official Symbol:TUBGCP2  
NCBI Official Synonym Symbols:GCP2; GCP-2; h103p; hGCP2; SPBC97; Spc97p; hSpc97; Grip103  
NCBI Protein Information:gamma-tubulin complex component 2
UniProt Protein Name:Gamma-tubulin complex component 2
UniProt Synonym Protein Names:Gamma-ring complex protein 103 kDa; h103p; hGrip103; Spindle pole body protein Spc97 homolog; hSpc97
UniProt Gene Name:TUBGCP2  
Anti-TUBGCP2 Antibody (CAB10283)Western blot analysis of extracts of various cell lines, using TUBGCP2 antibody (CAB10283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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