Cell Biology Antibodies 12
Anti-TUBB8 Antibody (CAB8396)
(No reviews yet)
Write a Review
- SKU:
- CAB8396
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TUBB8 Antibody |
| Antibody SKU: | CAB8396 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-444 of human TUBB8 (NP_817124.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Jurkat, K-562, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-444 of human TUBB8 (NP_817124.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GGGT GSGM GTLL LSKI REEY PDRI INTF SILP SPKV SDTV VEPY NATL SVHQ LIEN ADET FCID NEAL YDIC SKTL KLPT PTYG DLNH LVSA TMSG VTTC LRFP GQLN ADLR KLAV NMVP FPRL HFFM PGFA PLTS RGSQ QYRA LTVA ELTQ QMFD AKNM MAAC DPRH GRYL TAAA IFRG RMPM REVD EQMF NIQD KNSS YFAD WLPN NVKT AVCD IPPR GLKM SATF IGNN TAIQ ELFK RVSE QFTA MFRR KAFL HWYT GEGM DEME FTEA ESNM NDLV SEYQ QYQD ATAE EEED EEYA EEEV A |
| Gene ID: | 347688 |
| Uniprot: | Q3ZCM7 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 49kDa |
| Observed MW: | 50kDa |
| Synonyms: | TUBB8, OOMD, OOMD2, bA631M21.2 |
| Background: | The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility. |
| UniProt Protein Function: | TUBB8: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 10p15.3 |
| NCBI Summary: | The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q3ZCM7 |
| NCBI GenInfo Identifier: | 182705285 |
| NCBI Gene ID: | 347688 |
| NCBI Accession: | Q3ZCM7.2 |
| UniProt Secondary Accession: | Q3ZCM7,Q5SQX9, Q8WZ78, |
| UniProt Related Accession: | Q3ZCM7 |
| Molecular Weight: | 49,776 Da |
| NCBI Full Name: | Tubulin beta-8 chain |
| NCBI Synonym Full Names: | tubulin beta 8 class VIII |
| NCBI Official Symbol: | TUBB8 |
| NCBI Official Synonym Symbols: | OOMD; OOMD2; bA631M21.2 |
| NCBI Protein Information: | tubulin beta-8 chain |
| UniProt Protein Name: | Tubulin beta-8 chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBB8 |
| UniProt Entry Name: | TBB8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TUBB8 antibody (CAB8396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat lung using TUBB8 antibody (CAB8396) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using TUBB8 antibody (CAB8396) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using TUBB8 antibody (CAB8396) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक