Cell Biology Antibodies 9

Anti-TSTA3 Antibody (CAB4169)

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SKU:
CAB4169
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TSTA3 Antibody
Antibody SKU:CAB4169
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-321 of human TSTA3 (NP_003304.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, A-549, HepG2, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-321 of human TSTA3 (NP_003304.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGEP QGSM RILV TGGS GLVG KAIQ KVVA DGAG LPGE DWVF VSSK DADL TDTA QTRA LFEK VQPT HVIH LAAM VGGL FRNI KYNL DFWR KNVH MNDN VLHS AFEV GARK VVSC LSTC IFPD KTTY PIDE TMIH NGPP HNSN FGYS YAKR MIDV QNRA YFQQ YGCT FTAV IPTN VFGP HDNF NIED GHVL PGLI HKVH LAKS SGSA LTVW GTGN PRRQ FIYS LDLA QLFI WVLR EYNE VEPI ILSV GEED EVSI KEAA EAVV EAMD FHGE VTFD TTKS DGQF KKTA SNSK LRTY LPDF RFTP FKQA VKET CAWF TDNY EQAR K
Gene ID:7264
Uniprot:Q13630
Cellular Location:
Calculated MW:35kDa
Observed MW:36kDa
Synonyms:TSTA3, FX, P35B, SDR4E1
Background:Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.
UniProt Protein Function:TSTA3: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. Belongs to the fucose synthase family.
UniProt Protein Details:

Protein type:Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.271; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm

Molecular Function:GDP-L-fucose synthase activity; electron carrier activity; isomerase activity; coenzyme binding; GDP-4-dehydro-D-rhamnose reductase activity

Biological Process: leukocyte adhesion; cytolysis; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process

NCBI Summary:Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]
UniProt Code:Q13630
NCBI GenInfo Identifier:13124123
NCBI Gene ID:7264
NCBI Accession:Q13630.1
UniProt Secondary Accession:Q13630,Q567Q9, Q9UDG7, B2R8Y7, D3DWK5,
UniProt Related Accession:Q13630
Molecular Weight:321
NCBI Full Name:GDP-L-fucose synthase
NCBI Synonym Full Names:tissue specific transplantation antigen P35B
NCBI Official Symbol:TSTA3  
NCBI Official Synonym Symbols:FX; P35B; SDR4E1  
NCBI Protein Information:GDP-L-fucose synthase; 3-5 epimerase/4-reductase; red cell NADP(H)-binding protein; tissue specific transplantation antigen 3; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; short chain dehydrogenase/reductase family 4E, member 1
UniProt Protein Name:GDP-L-fucose synthase
UniProt Synonym Protein Names:GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; Protein FX; Red cell NADP(H)-binding protein; Short-chain dehydrogenase/reductase family 4E member 1
UniProt Gene Name:TSTA3  
UniProt Entry Name:FCL_HUMAN
Anti-TSTA3 Antibody (CAB4169)Western blot analysis of extracts of various cell lines, using TSTA3 antibody (CAB4169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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