Name: Anti-TSHR Antibody (CAB6781)
Brand: Cell Biology Antibodies 10
SKU: CAB6781
Availability: OutOfStock

Antibody Name:	Anti-TSHR Antibody
Antibody SKU:	CAB6781
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3, OVCAR3, mouse ovary, mouse thymus, mouse eye, rat ovary, rat thymus, rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GMGC SSPP CECH QEED FRVT CKDI QRIP SLPP STQT LKLI ETHL RTIP SHAF SNLP NISR IYVS IDVT LQQL ESHS FYNL SKVT HIEI RNTR NLTY IDPD ALKE LPLL KFLG IFNT GLKM FPDL TKVY STDI FFIL EITD NPYM TSIP VNAF QGLC NETL TLKL YNNG FTSV QGYA FNGT KLDA VYLN KNKY LTVI DKDA FGGV YSGP SLLL PLGR KSLS FETQ KAPR SSMP S
Gene ID:	7253
Uniprot:	P16473
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	28kDa/30kDa/86kDa
Observed MW:	110kDa

Synonyms:	TSHR, CHNG1, LGR3, hTSHR-I
Background:	The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TSHR: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH- independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation. Defects in TSHR are the cause of congenital hypothyroidism non-goitrous type 1 (CHNG1); also known as congenital hypothyroidism due to TSH resistance. CHNG1 is a non-autoimmune condition characterized by resistance to thyroid- stimulating hormone (TSH) leading to increased levels of plasma TSH and low levels of thyroid hormone. CHNG1 presents variable severity depending on the completeness of the defect. Most patients are euthyroid and asymptomatic, with a normal sized thyroid gland. Only a subset of patients develop hypothyroidism and present a hypoplastic thyroid gland. Defects in TSHR are the cause of familial gestational hyperthyroidism (HTFG). HTFG is a condition characterized by abnormally high levels of serum thyroid hormones occurring during early pregnancy. Defects in TSHR are the cause of hyperthyroidism non- autoimmune (HTNA). It is a condition characterized by abnormally high levels of serum thyroid hormones, thyroid hyperplasia, goiter and lack of anti-thyroid antibodies. Typical features of Graves disease such as exophthalmia, myxedema, antibodies anti-TSH receptor and lymphocytic infiltration of the thyroid gland are absent. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q31 Cellular Component: integral to plasma membrane; plasma membrane; receptor complex Molecular Function:protein binding; thyroid-stimulating hormone receptor activity Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; positive regulation of cell proliferation Disease: Hyperthyroidism, Nonautoimmune; Hypothyroidism, Congenital, Nongoitrous, 1; Hyperthyroidism, Familial Gestational
NCBI Summary:	The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
UniProt Code:	P16473
NCBI GenInfo Identifier:	62298994
NCBI Gene ID:	7253
NCBI Accession:	P16473.2
UniProt Secondary Accession:	P16473,Q16503, Q8TB90, Q96GT6, Q9P1V4, Q9ULA3, Q9UPH3 A0PJU7, F5GYU5, G3V2A9,
UniProt Related Accession:	P16473
Molecular Weight:	86,830 Da
NCBI Full Name:	Thyrotropin receptor
NCBI Synonym Full Names:	thyroid stimulating hormone receptor
NCBI Official Symbol:	TSHR
NCBI Official Synonym Symbols:	LGR3; CHNG1; hTSHR-I
NCBI Protein Information:	thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2
UniProt Protein Name:	Thyrotropin receptor
UniProt Synonym Protein Names:	Thyroid-stimulating hormone receptor
UniProt Gene Name:	TSHR
UniProt Entry Name:	TSHR_HUMAN

	Western blot analysis of extracts of various cell lines, using TSHR antibody (CAB6781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (CAB6781) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (CAB6781) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (CAB6781) at dilution of 1:100 (40x lens).