Cell Cycle Antibodies 1

Anti-TSG101 Antibody (CAB2216)

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SKU:
CAB2216
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-TSG101 Antibody
Antibody SKU:CAB2216
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101 (NP_006283.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, MCF7, K-562, Mouse spinal cord, Rat heart, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101 (NP_006283.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SALE KMEN QSEN NDID EVII PTAP LYKQ ILNL YAEE NAIE DTIF YLGE ALRR GVID LDVF LKHV RLLS RKQF QLRA LMQK ARKT AGLS DLY
Gene ID:7251
Uniprot:Q99816
Cellular Location:Cytoplasm, Late endosome membrane, Membrane, Nucleus, Peripheral membrane protein
Calculated MW:31kDa/43kDa
Observed MW:48kDa
Synonyms:TSG10, VPS23, TSG101 / VPS23, TSG101
Background:The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
UniProt Protein Function:TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: multivesicular body; late endosome membrane; early endosome; cytoplasm; late endosome; plasma membrane; nucleolus; endosome membrane

Molecular Function:ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer

NCBI Summary:The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
UniProt Code:Q99816
NCBI GenInfo Identifier:5454140
NCBI Gene ID:7251
NCBI Accession:NP_006283.1
UniProt Secondary Accession:Q99816,Q9BUM5,
UniProt Related Accession:Q99816
Molecular Weight:43,944 Da
NCBI Full Name:tumor susceptibility gene 101 protein
NCBI Synonym Full Names:tumor susceptibility gene 101
NCBI Official Symbol:TSG101  
NCBI Official Synonym Symbols:TSG10; VPS23  
NCBI Protein Information:tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101
UniProt Protein Name:Tumor susceptibility gene 101 protein
UniProt Synonym Protein Names:ESCRT-I complex subunit TSG101
Protein Family:Tumor susceptibility gene 101 protein
UniProt Gene Name:TSG101  
UniProt Entry Name:TS101_HUMAN
Anti-TSG101 Antibody (CAB2216)Western blot analysis of extracts of various cell lines, using TSG101 antibody (CAB2216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-TSG101 Antibody (CAB2216)Immunohistochemistry of paraffin-embedded Mouse kidney using TSG101 antibody (CAB2216) at dilution of 1:100 (40x lens).
Anti-TSG101 Antibody (CAB2216)Immunofluorescence analysis of L929 cells using TSG101 antibody (CAB2216) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-TSG101 Antibody (CAB2216)Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug TSG101 antibody (CAB2216). Western blot was performed from the immunoprecipitate using TSG101 antibody (CAB2216) at a dilition of 1:1000.
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