Epigenetics & Nuclear Signaling Antibodies 4

Anti-TSEN2 Antibody (CAB7838)

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SKU:
CAB7838
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TSEN2 Antibody
Antibody SKU:CAB7838
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TSEN2 (NP_079541.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HeLa, A-549, Mouse heart, Mouse brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TSEN2 (NP_079541.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEA VFHA PKRK RRVY ETYE SPLP IPFG QDHG PLKE FKIF RAEM INNN VIVR NAED IEQL YGKG YFGK GILS RSRP SFTI SDPK LVAK WKDM KTNM PIIT SKRY QHSV EWAA ELMR RQGQ DEST VRRI LKDY TKPL EHPP VKRN EEAQ VHDK LNSG MVSN MEGT AGGE RPSV VNGD SGKS GGVG DPRE PLGC LQEG SGCH PTTE SFEK SVRE DASP LPHV
Gene ID:80746
Uniprot:Q8NCE0
Cellular Location:Nucleus, nucleolus
Calculated MW:45kDa/47kDa/49kDa/51kDa/53kDa
Observed MW:53kDa
Synonyms:TSEN2, PCH2B, SEN2, SEN2L
Background:This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SEN2: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. Defects in TSEN2 are the cause of pontocerebellar hypoplasia type 2B (PCH2B). Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings. Belongs to the tRNA-intron endonuclease family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ribonuclease; EC 4.6.1.16; RNA processing; Nucleolus

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: centrosome; cytoplasm; nucleoplasm; tRNA-intron endonuclease complex

Molecular Function:protein binding; tRNA-intron endonuclease activity

Biological Process: tRNA splicing; tRNA-type intron splice site recognition and cleavage

Disease: Pontocerebellar Hypoplasia, Type 2b

NCBI Summary:This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
UniProt Code:Q8NCE0
NCBI GenInfo Identifier:50428914
NCBI Gene ID:80746
NCBI Accession:Q8NCE0.2
UniProt Secondary Accession:Q8NCE0,Q8WTW7, Q9BPU7, B7Z6K1, C9IZI7, G5E9Q3,
UniProt Related Accession:Q8NCE0
Molecular Weight:45,532 Da
NCBI Full Name:tRNA-splicing endonuclease subunit Sen2
NCBI Synonym Full Names:tRNA splicing endonuclease subunit 2
NCBI Official Symbol:TSEN2  
NCBI Official Synonym Symbols:SEN2; PCH2B; SEN2L  
NCBI Protein Information:tRNA-splicing endonuclease subunit Sen2
UniProt Protein Name:tRNA-splicing endonuclease subunit Sen2
UniProt Synonym Protein Names:tRNA-intron endonuclease Sen2; HsSen2
UniProt Gene Name:TSEN2  
UniProt Entry Name:SEN2_HUMAN
Anti-TSEN2 Antibody (CAB7838)Western blot analysis of extracts of various cell lines, using TSEN2 antibody (CAB7838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TSEN2 Antibody (CAB7838)Immunofluorescence analysis of A549 cells using TSEN2 antibody (CAB7838).
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