Immunology Antibodies 1
Anti-TSC2 Antibody (CAB0492)
- SKU:
- CAB0492
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TSC2 Antibody |
| Antibody SKU: | CAB0492 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1150 of human TSC2 (NP_000539.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1150 of human TSC2 (NP_000539.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CRLP FRKD FVPF ITKG LRSN VLLS FDDT PEKD SFRA RSTS LNER PKSL RIAR PPKQ GLNN SPPV KEFK ESSA AEAF RCRS ISVS EHVV RSRI QTSL TSAS LGSA DENS VAQA DDSL KNLH LELT ETCL DMMA RYVF SNFT AVPK RSPV GEFL LAGG RTKT WLVG NKLV TVTT SVGT GTRS LLGL DSGE LQSG PESS SSPG VHVR QTKE APAK LESQ AGQQ VSRG ARDR VRSM SGGH GLRV GALD VPAS QFL |
| Gene ID: | 7249 |
| Uniprot: | P49815 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 25kDa/187-200kDa |
| Observed MW: | 200KDa |
| Synonyms: | LAM, PPP1R160, TSC4, TSC2, Tuberin, tuberin |
| Background: | Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; GAP; GAP, misc.; Nuclear receptor co-regulator; Tumor suppressor Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: cytoplasm; cytosol; Golgi apparatus; lysosome; membrane; nucleus; perinuclear region of cytoplasm; TSC1-TSC2 complex Molecular Function:GTPase activator activity; phosphatase binding; protein binding; protein homodimerization activity; small GTPase binding Biological Process: anoikis; cell cycle arrest; endocytosis; heart development; insulin-like growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of protein kinase activity; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling pathway; negative regulation of Wnt receptor signaling pathway; neural tube closure; positive chemotaxis; positive regulation of macroautophagy; protein import into nucleus; protein kinase B signaling cascade; protein localization; regulation of cell cycle; regulation of endocytosis; regulation of insulin receptor signaling pathway; vesicle-mediated transport Disease: Focal Cortical Dysplasia Of Taylor; Lymphangioleiomyomatosis; Tuberous Sclerosis 2 |
| NCBI Summary: | Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49815 |
| NCBI GenInfo Identifier: | 269849475 |
| NCBI Gene ID: | 7249 |
| NCBI Accession: | P49815.2 |
| UniProt Secondary Accession: | P49815,O75275, Q4LE71, Q8TAZ1, A7E2E2, B4DIL8, B4DIQ7 B4DRN2, B7Z2B8, C9J378, |
| UniProt Related Accession: | P49815 |
| Molecular Weight: | 25,773 Da |
| NCBI Full Name: | Tuberin |
| NCBI Synonym Full Names: | tuberous sclerosis 2 |
| NCBI Official Symbol: | TSC2 |
| NCBI Official Synonym Symbols: | LAM; TSC4; PPP1R160 |
| NCBI Protein Information: | tuberin |
| UniProt Protein Name: | Tuberin |
| UniProt Synonym Protein Names: | Tuberous sclerosis 2 protein |
| Protein Family: | Tuberin |
| UniProt Gene Name: | TSC2 |
 | Western blot analysis of extracts of SH-SY5Y cells, using TSC2 antibody (CAB0492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat brain using TSC2 antibody (CAB0492) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using TSC2 antibody (CAB0492) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using TSC2 antibody (CAB0492) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of BALB-3T3 cells using TSC2 Rabbit pAb (CAB0492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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