Cell Biology Antibodies 1
Anti-TSC1 Antibody (CAB0720)
- SKU:
- CAB0720
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TSC1 Antibody |
| Antibody SKU: | CAB0720 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human TSC1 (NP_000359.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, LO2, Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human TSC1 (NP_000359.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QAFT PIDL PCGS ADES PAGD RECQ TSLE TSIF TPSP CKIP PPTR VGFG SGQP PPYD HLFE VALP KTAH HFVI RKTE ELLK KAKG NTEE DGVP STSP MEVL D |
| Gene ID: | 7248 |
| Uniprot: | Q92574 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 124kDa/129kDa |
| Observed MW: | 140-170kDa |
| Synonyms: | TSC1, LAM, TSC, hamartin |
| Background: | This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Tumor suppressor Chromosomal Location of Human Ortholog: 9q34 Cellular Component: TSC1-TSC2 complex; protein complex; growth cone; intracellular membrane-bound organelle; membrane; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol Molecular Function:protein binding; chaperone binding; protein N-terminus binding; GTPase activating protein binding; GTPase regulator activity Biological Process: myelination; protein heterooligomerization; regulation of cell-matrix adhesion; regulation of cell cycle; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; kidney development; cell cycle arrest; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; negative regulation of translation; regulation of stress fiber formation; glucose import; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1 |
| NCBI Summary: | This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009] |
| UniProt Code: | Q92574 |
| NCBI GenInfo Identifier: | 9297077 |
| NCBI Gene ID: | 7248 |
| NCBI Accession: | Q92574.2 |
| UniProt Secondary Accession: | Q92574,Q5VVN5, B7Z897, |
| UniProt Related Accession: | Q92574 |
| Molecular Weight: | 124,015 Da |
| NCBI Full Name: | Hamartin |
| NCBI Synonym Full Names: | tuberous sclerosis 1 |
| NCBI Official Symbol: | TSC1 |
| NCBI Official Synonym Symbols: | LAM; TSC |
| NCBI Protein Information: | hamartin; tumor suppressor; tuberous sclerosis 1 protein |
| UniProt Protein Name: | Hamartin |
| UniProt Synonym Protein Names: | Tuberous sclerosis 1 protein |
| Protein Family: | Hamartin |
| UniProt Gene Name: | TSC1 |
| UniProt Entry Name: | TSC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TSC1 antibody (CAB0720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat pancreatic islet using TSC1 antibody (CAB0720) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using TSC1 antibody (CAB0720) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreatic islet using TSC1 antibody (CAB0720) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using TSC1 Polyclonal Antibody (CAB0720) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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