Antibody Name:	Anti-TRPV1 Antibody
Antibody SKU:	CAB8564
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human TRPV1 (NP_061197.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, U-87MG, BxPC-3, Mouse pancreas, Mouse brain, Mouse skin, Rat brain, Rat skin

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human TRPV1 (NP_061197.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IAYS SSET PNRH DMLL VEPL NRLL QDKW DRFV KRIF YFNF LVYC LYMI IFTM AAYY RPVD GLPP FKME KTGD YFRV TGEI LSVL GGVY FFFR GIQY FLQR R
Gene ID:	7442
Uniprot:	Q8NER1
Cellular Location:	Cell junction, Cell membrane, Cell projection, Multi-pass membrane protein, dendritic spine membrane, postsynaptic cell membrane, synapse
Calculated MW:	94kDa
Observed MW:	112kDa

Synonyms:

TRPV1, VR1

Background:

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

UniProt Protein Function:	TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo.
UniProt Protein Details:	Protein type:Channel, cation; Membrane protein, multi-pass; Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: integral to membrane; integral to plasma membrane; intrinsic to plasma membrane; plasma membrane; postsynaptic membrane Molecular Function:ATP binding; calcium channel activity; calcium-release channel activity; excitatory extracellular ligand-gated ion channel activity; phosphoprotein binding; transmembrane receptor activity Biological Process: cell surface receptor linked signal transduction; chemosensory behavior; thermoception; transport
NCBI Summary:	Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8NER1
NCBI GenInfo Identifier:	296452849
NCBI Gene ID:	7442
NCBI Accession:	Q8NER1.2
UniProt Secondary Accession:	Q8NER1,Q3LU47, Q9H0G9, Q9H303, Q9H304, Q9NQ74, Q9NY22 A2RUA9,
UniProt Related Accession:	Q8NER1
Molecular Weight:	94,211 Da
NCBI Full Name:	Transient receptor potential cation channel subfamily V member 1
NCBI Synonym Full Names:	transient receptor potential cation channel subfamily V member 1
NCBI Official Symbol:	TRPV1
NCBI Official Synonym Symbols:	VR1
NCBI Protein Information:	transient receptor potential cation channel subfamily V member 1
UniProt Protein Name:	Transient receptor potential cation channel subfamily V member 1
UniProt Synonym Protein Names:	Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1
Protein Family:	Transient receptor potential cation channel subfamily
UniProt Gene Name:	TRPV1
UniProt Entry Name:	TRPV1_HUMAN

	Western blot analysis of extracts of various cell lines, using TRPV1 antibody (CAB8564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using TRPV1 antibody (CAB8564) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using TRPV1 antibody (CAB8564) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using TRPV1 antibody (CAB8564) at dilution of 1:100. Blue: DAPI for nuclear staining.