Signal Transduction Antibodies 1

Anti-TRPM8 Antibody (CAB12572)

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SKU:
CAB12572
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-TRPM8 Antibody
Antibody SKU:CAB12572
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 880-960 of human TRPM8 (NP_076985.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, 293T, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 880-960 of human TRPM8 (NP_076985.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AFGV ARQG ILRQ NEQR WRWI FRSV IYEP YLAM FGQV PSDV DGTT YDFA HCTF TGNE SKPL CVEL DEHN LPRF PEWI TIPL V
Gene ID:79054
Uniprot:Q7Z2W7
Cellular Location:Cell membrane, Endoplasmic reticulum membrane, Membrane raft, Multi-pass membrane protein
Calculated MW:5kDa/18kDa/79kDa/127kDa
Observed MW:122kDa
Synonyms:TRPM8, LTRPC6, TRPP8
Background:
UniProt Protein Function:TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: plasma membrane

Molecular Function:calcium channel activity; protein binding

UniProt Code:Q7Z2W7
NCBI GenInfo Identifier:143811469
NCBI Gene ID:79054
NCBI Accession:Q7Z2W7.2
UniProt Secondary Accession:Q7Z2W7,Q3YFM7, Q6QNH9, Q8TAC3, Q8TDX8, Q9BVK1, A0AVG2
UniProt Related Accession:Q7Z2W7
Molecular Weight:79,096 Da
NCBI Full Name:Transient receptor potential cation channel subfamily M member 8
NCBI Synonym Full Names:transient receptor potential cation channel subfamily M member 8
NCBI Official Symbol:TRPM8  
NCBI Official Synonym Symbols:TRPP8; LTRPC6  
NCBI Protein Information:transient receptor potential cation channel subfamily M member 8
UniProt Protein Name:Transient receptor potential cation channel subfamily M member 8
UniProt Synonym Protein Names:Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8
Protein Family:TRPM8 channel-associated factor
UniProt Gene Name:TRPM8  
UniProt Entry Name:TRPM8_HUMAN
Anti-TRPM8 Antibody (CAB12572)Western blot analysis of extracts of various cell lines, using TRPM8 antibody (CAB12572) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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