Immunology Antibodies 1

Anti-TRPM4 Antibody (CAB10146)

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SKU:
CAB10146
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-TRPM4 Antibody
Antibody SKU:CAB10146
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TRPM4 (NP_060106.2).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:1000 IHC 1:20 - 1:100
Reactivity:Human, Mouse
Positive Samples:22Rv1, HT-29, A-549, THP-1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TRPM4 (NP_060106.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVVP EKEQ SWIP KIFK KKTC TTFI VDST DPGG TLCQ CGRP RTAH PAVA MEDA FGAA VVTV WDSD AHTT EKPT DAYG ELDF TGAG RKHS NFLR LSDR TDPA AVYS LVTR TWGF RAPN LVVS VLGG SGGP VLQT WLQD LLRR GLVR AAQS TGAW IVTG GLHT GIGR HVGV AVRD HQMA STGG TKVV AMGV APWG VVRN RDTL INPK GSFP ARYR WRGD PEDG VQFP LDYN YSAF FLVD DGTH
Gene ID:54795
Uniprot:Q8TD43
Cellular Location:Cell membrane, Endoplasmic reticulum, Golgi apparatus, Golgi apparatus, Multi-pass membrane protein
Calculated MW:115kDa/118kDa/134kDa
Observed MW:150kDa
Synonyms:TRPM4, LTrpC4, PFHB1B, TRPM4B, hTRPM4
Background:The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium.
UniProt Protein Function:TRPM4: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Defects in TRPM4 are the cause of progressive familial heart block type 1B (PFHB1B). It is a cardiac bundle branch disorder characterized by progressive alteration of cardiac conduction through the His-Purkinje system, with a pattern of a right bundle-branch block and/or left anterior hemiblock occurring individually or together. It leads to complete atrioventricular block causing syncope and sudden death. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM4 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cell soma; cytosol; endoplasmic reticulum; Golgi apparatus; nucleoplasm; plasma membrane

Molecular Function:calcium activated cation channel activity; sodium channel activity

Biological Process: dendritic cell chemotaxis; elevation of cytosolic calcium ion concentration; negative regulation of bone mineralization; negative regulation of osteoblast differentiation; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of heart rate; positive regulation of vasoconstriction; protein sumoylation; regulation of T cell cytokine production

Disease: Progressive Familial Heart Block, Type Ib

NCBI Summary:The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. [provided by RefSeq, Mar 2016]
UniProt Code:Q8TD43
NCBI GenInfo Identifier:74715868
NCBI Gene ID:54795
NCBI Accession:Q8TD43.1
UniProt Secondary Accession:Q8TD43,Q7Z5D9, Q96L84, Q9NXV1, A2RU25,
UniProt Related Accession:Q8TD43
Molecular Weight:118,630 Da
NCBI Full Name:Transient receptor potential cation channel subfamily M member 4
NCBI Synonym Full Names:transient receptor potential cation channel subfamily M member 4
NCBI Official Symbol:TRPM4  
NCBI Official Synonym Symbols:LTrpC4; PFHB1B; TRPM4B; hTRPM4  
NCBI Protein Information:transient receptor potential cation channel subfamily M member 4
UniProt Protein Name:Transient receptor potential cation channel subfamily M member 4
UniProt Synonym Protein Names:Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; LTrpC-4; LTrpC4; Melastatin-4
Protein Family:Transient receptor potential cation channel subfamily
UniProt Gene Name:TRPM4  
UniProt Entry Name:TRPM4_HUMAN
Anti-TRPM4 Antibody (CAB10146)Western blot analysis of extracts of various cell lines, using TRPM4 antibody (CAB10146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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