Epigenetics & Nuclear Signaling Antibodies 4

Anti-TRMT61B Antibody (CAB8067)

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SKU:
CAB8067
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:TRMT61B Rabbit Polyclonal Antibody
Antibody SKU:CAB8067
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT61B (NP_060380.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT61B (NP_060380.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLMA WCRG PVLL CLRQ GLGT NSFL HGLG QEPF EGAR SLCC RSSP RDLR DGER EHEA AQRK APGA ESCP SLPL SISD IGTG CLSS LENL RLPT LREE SSPR ELED SSGD QGRC GPTH QGSE DPSM LSQA QSAT EVEE RHVS PSCS TSRE RPFQ AGEL ILAE TGEG ETKF KKLF RLNN FGLL NSNW GAVP FGKI VGKF PGQI LRSS FGKQ YMLR RPAL EDYV VLMK RGTA ITFP KDIN MILS MMDI NPGD TV
Gene ID:55006
Uniprot:Q9BVS5
Cellular Location:Mitochondrion
Calculated MW:52kDa
Observed MW:52kDa
Synonyms:TRMT61B
Background:
UniProt Protein Function:Methyltransferase that catalyzes the formation of N1-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (PubMed:23097428). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function (PubMed:27631568). In addition to tRNA N1-methyltransferase activity, also acts as a mRNA N1-methyltransferase by mediating methylation of adenosine residues at the N1 position of MT-ND5 mRNA, leading to interfere with mitochondrial translation (PubMed:29107537).
UniProt Code:Q9BVS5
NCBI GenInfo Identifier:222831587
NCBI Gene ID:55006
NCBI Accession:NP_060380.3
UniProt Secondary Accession:Q9BVS5,Q9H0Q9, Q9NWS7,
UniProt Related Accession:Q9BVS5
Molecular Weight:52,965 Da
NCBI Full Name:tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
NCBI Synonym Full Names:tRNA methyltransferase 61B
NCBI Official Symbol:TRMT61B  
NCBI Protein Information:tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
UniProt Protein Name:tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
UniProt Synonym Protein Names:mRNA methyladenosine-N(1)-methyltransferase
Protein Family:tRNA (adenine(58)-N(1))-methyltransferase
UniProt Gene Name:TRMT61B  
Anti-TRMT61B Polyclonal Antibody (CAB8067)Western blot analysis of extracts of mouse liver, using TRMT61B antibody (CAB8067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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