Epigenetics & Nuclear Signaling Antibodies 1

Anti-TRMT61A Antibody (CAB10597)

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SKU:
CAB10597
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TRMT61A Antibody
Antibody SKU:CAB10597
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, BT-474, U-251MG, Mouse brain, Mouse pancreas, Mouse spleen, Mouse liver, Rat testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSFV AYEE LIKE GDTA ILSL GHGA MVAV RVQR GAQT QTRH GVLR HSVD LIGR PFGS KVTC GRGG WVYV LHPT PELW TLNL PHRT QILY STDI ALIT MMLE LRPG SVVC ESGT GSGS VSHA IIRT IAPT GHLH TVEF HQQR AEKA REEF QEHR VGRW VTVR TQDV CRSG FGVS HVAD AVFL DIPS PWEA VGHA WDAL KVEG GRFC SFSP CIEQ VQRT CQAL AARG FSEL STLE VLPQ VYNV RTVS LPPP DLGT GTDG PAGS DTSP FRSG TPMK EAVG HTGY LTFA TKTP G
Gene ID:115708
Uniprot:Q96FX7
Cellular Location:Nucleus
Calculated MW:31kDa
Observed MW:31kDa
Synonyms:TRMT61A, C14orf172, GCD14, Gcd14p, TRM61, hTRM61
Background:
UniProt Protein Function:TRMT61A: Catalytic subunit of tRNA (adenine-N(1)-)- methyltransferase, which catalyzes the formation of N(1)- methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Belongs to the methyltransferase superfamily. TRM61 family.
UniProt Protein Details:

Protein type:EC 2.1.1.220; Methyltransferase

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: nucleoplasm; nucleus

Molecular Function:tRNA (adenine-N1-)-methyltransferase activity

Biological Process: tRNA modification

UniProt Code:Q96FX7
NCBI GenInfo Identifier:74760827
NCBI Gene ID:115708
NCBI Accession:Q96FX7.1
UniProt Secondary Accession:Q96FX7,Q8N7Q9, A6NN78,
UniProt Related Accession:Q96FX7
Molecular Weight:31kDa
NCBI Full Name:tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
NCBI Synonym Full Names:tRNA methyltransferase 61A
NCBI Official Symbol:TRMT61A  
NCBI Official Synonym Symbols:GCD14; TRM61; Gcd14p; hTRM61; C14orf172  
NCBI Protein Information:tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
UniProt Protein Name:tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
UniProt Synonym Protein Names:tRNA(m1A58)-methyltransferase subunit TRMT61A; tRNA(m1A58)MTase subunit TRMT61A
Protein Family:tRNA
UniProt Gene Name:TRMT61A  
Anti-TRMT61A Antibody (CAB10597)Western blot analysis of extracts of various cell lines, using TRMT61A antibody (CAB10597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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