Epigenetics & Nuclear Signaling Antibodies 4

Anti-TRMT6 Antibody (CAB9212)

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SKU:
CAB9212
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TRMT6 Antibody
Antibody SKU:CAB9212
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TRMT6 (NP_057023.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TRMT6 (NP_057023.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEGS GEQP GPQP QHPG DHRI RDGD FVVL KRED VFKA VQVQ RRKK VTFE KQWF YLDN VIGH SYGT AFEV TSGG SLQP KKKR EEPT AETK EAGT DNRN IVDD GKSQ KLTQ DDIK ALKD KGIK GEEI VQQL IENS TTFR DKTE FAQD KYIK KKKK KYEA IITV VKPS TRIL SIMY YARE PGKI NHMR YDTL AQML TLGN IRAG
Gene ID:51605
Uniprot:Q9UJA5
Cellular Location:Nucleus
Calculated MW:36kDa/40kDa/44kDa/55kDa
Observed MW:60kDa
Synonyms:TRMT6, CGI-09, GCD10, Gcd10p
Background:This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants.
UniProt Code:Q9UJA5
NCBI GenInfo Identifier:527317379
NCBI Gene ID:51605
NCBI Accession:NP_001268396.1
UniProt Secondary Accession:Q9UJA5,Q76P92, Q9BQV5, Q9ULR7, Q9Y2Z8, B4DUV6,
UniProt Related Accession:Q9UJA5
Molecular Weight:55,799 Da
NCBI Full Name:tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 isoform 2
NCBI Synonym Full Names:tRNA methyltransferase 6 homolog (S. cerevisiae)
NCBI Official Symbol:TRMT6  
NCBI Official Synonym Symbols:GCD10; CGI-09; Gcd10p  
NCBI Protein Information:tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6; tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6; tRNA(m1A58)MTase subunit TRM6
UniProt Protein Name:tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
UniProt Synonym Protein Names:tRNA(m1A58)-methyltransferase subunit TRM6; tRNA(m1A58)MTase subunit TRM6
UniProt Gene Name:TRMT6  
UniProt Entry Name:TRM6_HUMAN
Anti-TRMT6 Polyclonal Antibody (CAB9212)Western blot analysis of extracts of various cell lines, using TRMT6 antibody (CAB9212) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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