Epigenetics & Nuclear Signaling Antibodies 3
Anti-TRMT112 Antibody (CAB14310)
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- SKU:
- CAB14310
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TRMT112 Antibody |
| Antibody SKU: | CAB14310 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human TRMT112 (NP_057488.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, DU145, HeLa, Jurkat, Mouse brain, Mouse kidney, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human TRMT112 (NP_057488.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKLL THNL LSSH VRGV GSRG FPLR LQAT EVRI CPVE FNPN FVAR MIPK VEWS AFLE AADN LRLI QVPK GPVE GYEE NEEF LRTM HHLL LEVE VIEG TLQC PESG RMFP ISRG IPNM LLSE EETE S |
| Gene ID: | 51504 |
| Uniprot: | Q9UI30 |
| Cellular Location: | |
| Calculated MW: | 13kDa/14kDa |
| Observed MW: | 14kDa |
| Synonyms: | TRMT112, HSPC152, HSPC170, TRM112, TRMT11-2, hTrm112 |
| Background: |
| UniProt Protein Function: | HSPC152: Participates both in methylation of protein and tRNA species. The heterodimer with HEMK2/N6AMT1 catalyzes N5- methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species. Belongs to the TRM112 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytosol; nucleoplasm; protein complex Molecular Function:protein binding; protein methyltransferase activity Biological Process: methylation; peptidyl-glutamine methylation; rRNA methylation; translational termination; tRNA methylation |
| UniProt Code: | Q9UI30 |
| NCBI GenInfo Identifier: | 554506493 |
| NCBI Gene ID: | 51504 |
| NCBI Accession: | NP_001273011.1 |
| UniProt Secondary Accession: | Q9UI30,Q3MHC7, Q8N2Z4, B2R539, J3KNG5, |
| UniProt Related Accession: | Q9UI30 |
| Molecular Weight: | 30.2kD |
| NCBI Full Name: | multifunctional methyltransferase subunit TRM112-like protein isoform 2 |
| NCBI Synonym Full Names: | tRNA methyltransferase subunit 11-2 |
| NCBI Official Symbol: | TRMT112 |
| NCBI Official Synonym Symbols: | TRM112; HSPC152; HSPC170; hTrm112; TRMT11-2 |
| NCBI Protein Information: | multifunctional methyltransferase subunit TRM112-like protein |
| UniProt Protein Name: | Multifunctional methyltransferase subunit TRM112-like protein |
| UniProt Synonym Protein Names: | tRNA methyltransferase 112 homolog |
| UniProt Gene Name: | TRMT112 |
 | Western blot analysis of extracts of various cell lines, using TRMT112 antibody (CAB14310) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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