Developmental Biology
Anti-TrkB Antibody (CAB12011)
- SKU:
- CAB12011
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-TrkB Antibody |
| Antibody SKU: | CAB12011 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 41-433 of human TrkB (NP_001007098.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | K-562, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 41-433 of human TrkB (NP_001007098.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SRIW CSDP SPGI VAFP RLEP NSVD PENI TEIF IANQ KRLE IINE DDVE AYVG LRNL TIVD SGLK FVAH KAFL KNSN LQHI NFTR NKLT SLSR KHFR HLDL SELI LVGN PFTC SCDI MWIK TLQE AKSS PDTQ DLYC LNES SKNI PLAN LQIP NCGL PSAN LAAP NLTV EEGK SITL SCSV AGDP VPNM YWDV GNLV SKHM NETS HTQG SLRI TNIS SDDS GKQI SCVA ENLV GEDQ DSVN LTVH FAPT ITFL ESPT SDHH WCIP FTVK GNPK PALQ WFYN GAIL NESK YICT KIHV TNHT EYHG CLQL DNPT HMNN GDYT LIAK NEYG KDEK QISA HFMG WPGI DDGA NPNY PDVI YEDY GTAA NDIG DTTN RSNE IPST DVTD KTGR EHLS V |
| Gene ID: | 4915 |
| Uniprot: | Q16620 |
| Cellular Location: | Cell membrane, Endosome membrane, Single-pass type I membrane protein |
| Calculated MW: | 35kDa/53kDa/59kDa/60kDa/81kDa/91kDa/93kDa |
| Observed MW: | 110kDa |
| Synonyms: | NTRK2, GP145-TrkB, TRKB, trk-B, TrkB, OBHD |
| Background: | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Trk family Chromosomal Location of Human Ortholog: 9q22.1 Cellular Component: cell surface; rough endoplasmic reticulum; integral to plasma membrane; dendritic spine; terminal button; excitatory synapse; perikaryon; cytosol; postsynaptic membrane; growth cone; presynaptic active zone; endosome membrane; receptor complex Molecular Function:brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding Biological Process: circadian rhythm; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; retinal rod cell development; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; neuron migration; regulation of dendrite development; regulation of neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; central nervous system neuron development; aging; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; peripheral nervous system neuron development; long-term memory; glutamate secretion; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway Disease: Obesity, Hyperphagia, And Developmental Delay |
| NCBI Summary: | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | Q16620 |
| NCBI GenInfo Identifier: | 2497560 |
| NCBI Gene ID: | 4915 |
| NCBI Accession: | Q16620.1 |
| UniProt Secondary Accession: | Q16620,Q16675, Q59GJ1, Q8WXJ5, Q8WXJ6, Q8WXJ7, B1ANZ4 B4DFV9, |
| UniProt Related Accession: | Q16620 |
| Molecular Weight: | 822 |
| NCBI Full Name: | BDNF/NT-3 growth factors receptor |
| NCBI Synonym Full Names: | neurotrophic tyrosine kinase, receptor, type 2 |
| NCBI Official Symbol: | NTRK2 |
| NCBI Official Synonym Symbols: | TRKB; trk-B; GP145-TrkB |
| NCBI Protein Information: | BDNF/NT-3 growth factors receptor; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B |
| UniProt Protein Name: | BDNF/NT-3 growth factors receptor |
| UniProt Synonym Protein Names: | GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B |
| Protein Family: | BDNF/NT-3 growth factors receptor |
| UniProt Gene Name: | NTRK2 |
| UniProt Entry Name: | NTRK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TrkB Antibody (CAB12011) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using TrkB antibody (CAB12011) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using TrkB antibody (CAB12011) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using TrkB antibody (CAB12011) at dilution of 1:200 (40x lens). |
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