Name: Anti-TRIP12 Antibody (CAB9958)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB9958
Availability: OutOfStock

Antibody Name:	Anti-TRIP12 Antibody
Antibody SKU:	CAB9958
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TRIP12 (NP_001271143.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TRIP12 (NP_001271143.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSNR PNNN PGGS LRRS QRNT AGAQ PQDD SIGG RSHL GQAK HKGY SPPE SRKS NSKA PKVQ SNTT SELS RGHL SKRS CSSS SAVI VPQP EDPD RANT SERQ KTGQ VPKK DNSR GVKR SASP DYNR TNSP SSAK KPKA LQHT ESPS ETNK PHSK SKKR HLDQ EQQL KSAQ SPST SKAH TRKS GATG GSRS QKRK RTES SCVK SGSG SEST GAEE RSAK PTKL ASKS ATSA KAGC STIT DSSS
Gene ID:	9320
Uniprot:	Q14669
Cellular Location:	Nucleus, nucleoplasm
Calculated MW:	192kDa/220kDa/223kDa/225kDa
Observed MW:	260kDa

Synonyms:	TRIP12, TRIP-12, ULF
Background:	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53.

UniProt Protein Function:	TRIP12: E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. Belongs to the UPL family. K-HECT subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin ligase; Ligase; EC 6.3.2.-; Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 2q36.3 Cellular Component: nucleoplasm; nucleus Molecular Function:ligase activity; protein binding; thyroid hormone receptor binding; ubiquitin-protein ligase activity Biological Process: DNA repair; embryonic development; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to DNA damage stimulus
UniProt Code:	Q14669
NCBI GenInfo Identifier:	2499839
NCBI Gene ID:	9320
NCBI Accession:	Q14669.1
UniProt Secondary Accession:	Q14669,Q14CA3, Q14CF1, Q15644, Q53R87, Q53TE7, D4HL82
UniProt Related Accession:	Q14669
Molecular Weight:	192,075 Da
NCBI Full Name:	E3 ubiquitin-protein ligase TRIP12
NCBI Synonym Full Names:	thyroid hormone receptor interactor 12
NCBI Official Symbol:	TRIP12
NCBI Official Synonym Symbols:	ULF; TRIP-12
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIP12
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIP12
UniProt Synonym Protein Names:	E3 ubiquitin-protein ligase for Arf; ULF; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	TRIP12
UniProt Entry Name:	TRIPC_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIP12 antibody (CAB9958) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human colon using TRIP12 antibody (CAB9958) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using TRIP12 antibody (CAB9958) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using TRIP12 antibody (CAB9958) at dilution of 1:100 (40x lens).