Anti-TriMethyl-Histone H3-K36 Antibody (CAB20379)
- SKU:
- CAB20379
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Applications:
- WB
- Applications:
- IP
- Applications:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- H3.4
- Synonyms:
- H3/g
- Synonyms:
- H3FT
- Synonyms:
- H3t
- Synonyms:
- HIST3H3
- Synonyms:
- Histone H3
- Synonyms:
- HIST1H3A
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Product Name: | TriMethyl-Histone H3-K36 Rabbit mAb |
| Product Code: | CAB20379 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| Applications: | WB, IP, ChIP, DB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1). |
| Applications: | WB, IP, ChIP, DB |
| Recommended Dilutions: | DB 1:500 - 1:2000 WB 1:500 - 1:2000 ChIP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Immunogen: | A synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1). |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8290/ 8350 |
| Uniprot: | Q16695/P68431 |
| Calculated MW: | 15kDa |
| Observed MW: | 17kDa |
| UniProt Protein Function: | HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16695 |
| NCBI GenInfo Identifier: | 18202512 |
| NCBI Gene ID: | 8290 |
| NCBI Accession: | Q16695.3 |
| UniProt Secondary Accession: | Q16695,Q6FGU4, B2R5K3, |
| UniProt Related Accession: | Q16695 |
| Molecular Weight: | |
| NCBI Full Name: | Histone H3.1t |
| NCBI Synonym Full Names: | histone cluster 3, H3 |
| NCBI Official Symbol: | HIST3H3 |
| NCBI Official Synonym Symbols: | H3t; H3.4; H3/g; H3FT |
| NCBI Protein Information: | histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T |
| UniProt Protein Name: | Histone H3.1t |
| UniProt Synonym Protein Names: | H3/g |
| UniProt Gene Name: | HIST3H3 |
| UniProt Entry Name: | H31T_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K36 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
 | Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K36 antibody at 1:1000 dilution. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K36 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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