Epigenetics & Nuclear Signaling Antibodies 2
Anti-TriMethyl-Histone H3-K27 Antibody (CAB16199)
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- SKU:
- CAB16199
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Applications:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Mouse
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TriMethyl-Histone H3-K27 Mouse Monoclonal Antibody |
| Antibody SKU: | CAB16199 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP ChIP |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Host Species: | Mouse |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Application: | WB IHC IF IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Positive Samples: | HepG2, U-87MG, A-375, NIH/3T3, C2C12, C6 |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8290 |
| Uniprot: | Q16695 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
| Synonyms: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| UniProt Protein Function: | HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q42 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16695 |
| NCBI GenInfo Identifier: | 18202512 |
| NCBI Gene ID: | 8290 |
| NCBI Accession: | Q16695.3 |
| UniProt Secondary Accession: | Q16695,Q6FGU4, B2R5K3, |
| UniProt Related Accession: | Q16695 |
| Molecular Weight: | |
| NCBI Full Name: | Histone H3.1t |
| NCBI Synonym Full Names: | histone cluster 3, H3 |
| NCBI Official Symbol: | HIST3H3 |
| NCBI Official Synonym Symbols: | H3t; H3.4; H3/g; H3FT |
| NCBI Protein Information: | histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T |
| UniProt Protein Name: | Histone H3.1t |
| UniProt Synonym Protein Names: | H3/g |
| UniProt Gene Name: | HIST3H3 |
| UniProt Entry Name: | H31T_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
 | Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody at dilution of 1:25 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody at dilution of 1:25 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody at dilution of 1:25 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:25. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:25. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody at dilution of 1:25. Blue: DAPI for nuclear staining. |
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