Name: Anti-TRIM9 Antibody (CAB13895)
Brand: Signal Transduction Antibodies 1
SKU: CAB13895
Availability: OutOfStock

Antibody Name:	Anti-TRIM9 Antibody
Antibody SKU:	CAB13895
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TRIM9 (NP_055978.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TRIM9 (NP_055978.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEM EEEL KCPV CGSF YREP IILP CSHN LCQA CARN ILVQ TPES ESPQ SHRA AGSG VSDY DYLD LDKM SLYS EADS GYGS YGGF ASAP TTPC QKSP NGVR VFPP AMPP PATH LSPA LAPV PRNS CITC PQCH RSLI LDDR GLRG FPKN RVLE GVID RYQQ
Gene ID:	114088
Uniprot:	Q9C026
Cellular Location:	Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, cytoskeleton, dendrite, secretory vesicle, synapse, synaptic vesicle
Calculated MW:	61kDa/79kDa/89kDa
Observed MW:	65kDa

Synonyms:	TRIM9, RNF91, SPRING
Background:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.

UniProt Protein Function:	TRIM9: E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; EC 6.3.2.- Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: synaptic vesicle; cytoskeleton; dendrite; cytoplasm; cell junction Molecular Function:SNARE binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; synaptic vesicle exocytosis; negative regulation of calcium ion-dependent exocytosis; protein ubiquitination
NCBI Summary:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9C026
NCBI GenInfo Identifier:	39795457
NCBI Gene ID:	114088
NCBI Accession:	AAH63872.1
UniProt Secondary Accession:	Q9C026,Q92557, Q96D24, Q96NI4, Q9C025, Q9C027, D3DSB7 D3DSB8,
UniProt Related Accession:	Q9C026
Molecular Weight:
NCBI Full Name:	Tripartite motif-containing 9
NCBI Synonym Full Names:	tripartite motif containing 9
NCBI Official Symbol:	TRIM9
NCBI Official Synonym Symbols:	RNF91; SPRING
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM9; RING finger protein 91; tripartite motif-containing 9; homolog of rat RING finger Spring; tripartite motif-containing protein 9
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM9
UniProt Synonym Protein Names:	RING finger protein 91; Tripartite motif-containing protein 9
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	TRIM9
UniProt Entry Name:	TRIM9_HUMAN

Western blot analysis of extracts of various cell lines, using TRIM9 antibody (CAB13895) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-TRIM9 Antibody (CAB13896)

TRIM9 Antibody (PACO12925)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)