Cell Biology Antibodies 4

Anti-TRIM59 Antibody (CAB13876)

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SKU:
CAB13876
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TRIM59 Antibody
Antibody SKU:CAB13876
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human TRIM59 (NP_775107.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, NIH/3T3, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human TRIM59 (NP_775107.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MHNF EEEL TCPI CYSI FEDP RVLP CSHT FCRN CLEN ILQA SGNF YIWR PLRI PLKC PNCR SITE IAPT GIES LPVN FALR AIIE KYQQ EDHP DIVT CPEH YRQP LNVY CLLD KKLV CGHC LTIG QHHG HPID DLQS AYLK EKDT PQKL LEQL TDTH WTDL THLI EKLK EQKS HSEK MIQG DKEA VLQY FKEL NDTL EQKK KSFL TALC DVGN LINQ EYTP QIER MKEI REQQ LELM ALTI SLQE ESPL KFLE KVDD VRQH VQIL KQRP LPEV QPVE IYPR VSKI LKEE WSRT EIGQ IKNV LIPK MKIS PKRM SCSW PGKD EKEV EFLK IL
Gene ID:286827
Uniprot:Q8IWR1
Cellular Location:Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:47kDa/123kDa
Observed MW:50kDa
Synonyms:TRIM59, IFT80L, MRF1, RNF104, TRIM57, TSBF1
Background:
UniProt Protein Function:TRIM59: Belongs to the TRIM/RBCC family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: centrosome; cilium; endoplasmic reticulum

Molecular Function:protein binding

Biological Process: cilium biogenesis; negative regulation of I-kappaB kinase/NF-kappaB cascade

UniProt Code:Q8IWR1
NCBI GenInfo Identifier:74714421
NCBI Gene ID:286827
NCBI Accession:Q8IWR1.1
UniProt Secondary Accession:Q8IWR1,A8K5G9, D3DNL9,
UniProt Related Accession:Q8IWR1
Molecular Weight:123,321 Da
NCBI Full Name:Tripartite motif-containing protein 59
NCBI Synonym Full Names:tripartite motif containing 59
NCBI Official Symbol:TRIM59  
NCBI Official Synonym Symbols:MRF1; TSBF1; IFT80L; RNF104; TRIM57  
NCBI Protein Information:tripartite motif-containing protein 59
UniProt Protein Name:Tripartite motif-containing protein 59
UniProt Synonym Protein Names:RING finger protein 104; Tumor suppressor TSBF-1
Protein Family:Tripartite motif-containing protein
UniProt Gene Name:TRIM59  
UniProt Entry Name:TRI59_HUMAN
Anti-TRIM59 Antibody (CAB13876)Western blot analysis of extracts of various cell lines, using TRIM59 antibody (CAB13876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TRIM59 Antibody (CAB13876)Western blot analysis of extracts of various cell lines, using TRIM59 antibody (CAB13876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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