Cell Biology Antibodies 6

Anti-TRIM55 Antibody (CAB15917)

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SKU:
CAB15917
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TRIM55 Antibody
Antibody SKU:CAB15917
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 183-452 of human TRIM55 (NP_908974.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, HepG2, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 183-452 of human TRIM55 (NP_908974.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NDRV QGVI SQLE DTCK TIEE CCRK QKQE LCEK FDYL YGIL EERK NEMT QVIT RTQE EKLE HVRA LIKK YSDH LENV SKLV ESGI QFMD EPEM AVFL QNAK TLLK KISE ASKA FQME KIEH GYEN MNHF TVNL NREE KIIR EIDF YRED EDEE EEEG GEGE KEGE GEVG GEAV EVEE VENV QTEF PGED ENPE KASE LSQV ELQA APGA LPVS SPEP PPAL PPAA DAPV TQIG FEAP PLQG QAAA PASG SGAD SEPA RHIF SFSW LNSL NE
Gene ID:84675
Uniprot:Q9BYV6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:26kDa/50kDa/60kDa
Observed MW:60kDa
Synonyms:TRIM55, MURF-2, RNF29, muRF2
Background:The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described.
UniProt Protein Function:TRIM55: May regulate gene expression and protein turnover in muscle cells. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 8q13.1

Molecular Function:identical protein binding; protein binding

NCBI Summary:The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BYV6
NCBI GenInfo Identifier:78099806
NCBI Gene ID:84675
NCBI Accession:Q9BYV6.2
UniProt Secondary Accession:Q9BYV6,Q53XX3, Q8IUD9, Q8IUE4, Q96DV2, Q96DV3, Q9BYV5 B3KRC0, B3KRJ3,
UniProt Related Accession:Q9BYV6
Molecular Weight:26,841 Da
NCBI Full Name:Tripartite motif-containing protein 55
NCBI Synonym Full Names:tripartite motif containing 55
NCBI Official Symbol:TRIM55  
NCBI Official Synonym Symbols:RNF29; muRF2; MURF-2  
NCBI Protein Information:tripartite motif-containing protein 55
UniProt Protein Name:Tripartite motif-containing protein 55
UniProt Synonym Protein Names:Muscle-specific RING finger protein 2; MuRF-2; MuRF2; RING finger protein 29
Protein Family:Tripartite motif-containing protein
UniProt Gene Name:TRIM55  
UniProt Entry Name:TRI55_HUMAN
Anti-TRIM55 Antibody (CAB15917)Western blot analysis of extracts of various cell lines, using TRIM55 antibody (CAB15917) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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