Name: Anti-TRIM54 Antibody (CAB13111)
Brand: Developmental Biology
SKU: CAB13111
Availability: OutOfStock

Antibody Name:	Anti-TRIM54 Antibody
Antibody SKU:	CAB13111
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human TRIM54 (NP_912730.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human TRIM54 (NP_912730.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AMLV AGND RVQA VITQ MEEV CQTI EDNS RRQK QLLN QRFE SLCA VLEE RKGE LLQA LARE QEEK LQRV RGLI RQYG DHLE ASSK LVES AIQS MEEP QMAL YLQQ AKEL INKV GAMS KVEL AGRP EPGY ESME QFTV RVEH VAEM LRTI DFQP GASG EEEE VAPD GEEG SAGP EEER PDGP
Gene ID:	57159
Uniprot:	Q9BYV2
Cellular Location:	Cytoplasm, Z line, cytoskeleton, myofibril, sarcomere
Calculated MW:	40kDa/44kDa
Observed MW:	40kDa

Synonyms:

TRIM54, MURF, MURF-3, RNF30, muRF3

Background:

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	TRIM54: May bind and stabilize microtubules during myotubes formation. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: microtubule; microtubule associated complex; Z disc Molecular Function:signal transducer activity; zinc ion binding; microtubule binding Biological Process: negative regulation of microtubule depolymerization; multicellular organismal development; signal transduction; cell differentiation; microtubule-based process
NCBI Summary:	The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BYV2
NCBI GenInfo Identifier:	209572715
NCBI Gene ID:	57159
NCBI Accession:	Q9BYV2.3
UniProt Secondary Accession:	Q9BYV2,Q53SY4, Q9BYV3, A5D8T7,
UniProt Related Accession:	Q9BYV2
Molecular Weight:	358
NCBI Full Name:	Tripartite motif-containing protein 54
NCBI Synonym Full Names:	tripartite motif containing 54
NCBI Official Symbol:	TRIM54
NCBI Official Synonym Symbols:	MURF; RNF30; muRF3; MURF-3
NCBI Protein Information:	tripartite motif-containing protein 54; ring finger protein 30; tripartite motif-containing 54; muscle-specific RING finger protein 3; muscle-specific RING-finger protein 3
UniProt Protein Name:	Tripartite motif-containing protein 54
UniProt Synonym Protein Names:	Muscle-specific RING finger protein; MuRF; Muscle-specific RING finger protein 3; MuRF-3; MuRF3; RING finger protein 30
Protein Family:	Tripartite motif-containing protein
UniProt Gene Name:	TRIM54
UniProt Entry Name:	TRI54_HUMAN

Western blot analysis of extracts of various cell lines, using TRIM54 antibody (CAB13111) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

TRIM54 Antibody (PACO12922)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)