Immunology Antibodies 2
Anti-TRIM5 Antibody (CAB1872)
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- SKU:
- CAB1872
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TRIM5 Antibody |
| Antibody SKU: | CAB1872 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Jurkat, HepG2, Mouse stomach, Mouse thymus |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EKLL LFCQ EDGK VICW LCER SQEH RGHH TFLT EEVA REYQ VKLQ AALE MLRQ KQQE AEEL EADI REEK ASWK TQIQ YDKT NVLA DFEQ LRDI LDWE ESNE L |
| Gene ID: | 85363 |
| Uniprot: | Q9C035 |
| Cellular Location: | Cytoplasm, Nucleus, P-body |
| Calculated MW: | 29kDa/31kDa/37kDa/40kDa/46kDa/56kDa |
| Observed MW: | 50kDa |
| Synonyms: | TRIM5, RNF88, TRIM5alpha |
| Background: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Ubiquitin ligase Chromosomal Location of Human Ortholog: 11p15 Cellular Component: cytoplasm; cytosol Molecular Function:identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of MAPKKK cascade; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; activation of innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; defense response to virus; activation of NF-kappaB transcription factor |
| NCBI Summary: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Code: | Q9C035 |
| NCBI GenInfo Identifier: | 283046694 |
| NCBI Gene ID: | 85363 |
| NCBI Accession: | NP_149023.2 |
| UniProt Secondary Accession: | Q9C035,Q2MLV4, Q2MLV8, Q2MLV9, Q2MLW1, Q2MLW3, Q2MLW4 A6NGQ1, A8WFA8, D3DQS8, D3DQS9, G3GJY1, |
| UniProt Related Accession: | Q9C035 |
| Molecular Weight: | 70.3 kDa |
| NCBI Full Name: | tripartite motif-containing protein 5 isoform alpha |
| NCBI Synonym Full Names: | tripartite motif containing 5 |
| NCBI Official Symbol: | TRIM5 |
| NCBI Official Synonym Symbols: | RNF88; TRIM5alpha |
| NCBI Protein Information: | tripartite motif-containing protein 5; ring finger protein 88; tripartite motif protein TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa |
| UniProt Protein Name: | Tripartite motif-containing protein 5 |
| UniProt Synonym Protein Names: | RING finger protein 88 |
| Protein Family: | Tripartite motif-containing protein |
| UniProt Gene Name: | TRIM5 |
| UniProt Entry Name: | TRIM5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRIM5 antibody (CAB1872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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