Cell Biology Antibodies 3
Anti-TRIM45 Antibody (CAB12739)
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- SKU:
- CAB12739
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TRIM45 Antibody |
| Antibody SKU: | CAB12739 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM45 (NP_001139107.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM45 (NP_001139107.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QVDL PMGG VKAL TIDH LAVN DVML ESLR GEGQ GLVC DLCN DREV EKRC QTCK ANLC HFCC QAHR RQKK TTYH TMVD LKDL KGYS RIGK PILC PVHP AEEL RLFC EFCD RPVC QDCV VGEH REHP CDFT SNVI HKHG DSVW ELLK GTQP HVEA LEEA LAQI HIIN SALQ KRVE AVAA DVRT FSEG YIKA IEEH RDKL LKQL EDIR AQKE NSLQ LQKA QLEQ LLAD MRTG VEFT EHLL TSGS DLEI LITK RVVV ERLR KLNK VQYS TRPG VNDK IRFC PQEK AGQC RGYE IYDL HRAR EKQT A |
| Gene ID: | 80263 |
| Uniprot: | Q9H8W5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 62kDa/64kDa |
| Observed MW: | 64KDa |
| Synonyms: | TRIM45, RNF99 |
| Background: | This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | TRIM45: May act as a transcriptional repressor in mitogen- activated protein kinase signaling pathway. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: cytoplasm; intercellular bridge; nucleoplasm |
| NCBI Summary: | This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] |
| UniProt Code: | Q9H8W5 |
| NCBI GenInfo Identifier: | 90110721 |
| NCBI Gene ID: | 80263 |
| NCBI Accession: | Q9H8W5.2 |
| UniProt Secondary Accession: | Q9H8W5,Q53GN0, Q5T2K4, Q5T2K5, Q8IYV6, |
| UniProt Related Accession: | Q9H8W5 |
| Molecular Weight: | 62,475 Da |
| NCBI Full Name: | Tripartite motif-containing protein 45 |
| NCBI Synonym Full Names: | tripartite motif containing 45 |
| NCBI Official Symbol: | TRIM45 |
| NCBI Official Synonym Symbols: | RNF99 |
| NCBI Protein Information: | tripartite motif-containing protein 45 |
| UniProt Protein Name: | Tripartite motif-containing protein 45 |
| UniProt Synonym Protein Names: | RING finger protein 99 |
| Protein Family: | Tripartite motif-containing protein |
| UniProt Gene Name: | TRIM45 |
| UniProt Entry Name: | TRI45_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using TRIM45 Rabbit pAb (CAB12739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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