Immunology Antibodies 3

Anti-TRIM23 Antibody (CAB8329)

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SKU:
CAB8329
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-TRIM23 Antibody
Antibody SKU:CAB8329
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM23 (NP_001647.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-12, Mouse spleen, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TRIM23 (NP_001647.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATL VVNK LGAG VDSG RQGS RGTA VVKV LECG VCED VFSL QGDK VPRL LLCG HTVC HDCL TRLP LHGR AIRC PFDR QVTD LGDS GVWG LKKN FALL ELLE RLQN GPIG QYGA AEES IGIS GESI IRCD EDEA HLAS VYCT VCAT HLCS ECSQ VTHS TKTL AKHR RVPL ADKP HEKT MCSQ HQVH AIEF VCLE EGCQ TSPL MCCV CKEY GKHQ GHKH SVLE PEAN QIRA SILD MAHC IRTF TEEI SDYS RKLV GIVQ HIEG GEQI VEDG IGMA HTEH VPGT
Gene ID:373
Uniprot:P36406
Cellular Location:Endomembrane system, Golgi apparatus membrane, Lysosome membrane
Calculated MW:61kDa/63kDa/64kDa
Observed MW:64kDa
Synonyms:TRIM23, ARD1, ARFD1, RNF46
Background:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described.
UniProt Protein Function:TRIM23: Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: Golgi membrane; lysosomal membrane

Molecular Function:enzyme activator activity; GDP binding; GTP binding; GTPase activity; identical protein binding; protein binding; ubiquitin-protein ligase activity

Biological Process: protein ubiquitination

NCBI Summary:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq, Jul 2008]
UniProt Code:P36406
NCBI GenInfo Identifier:543839
NCBI Gene ID:373
NCBI Accession:P36406.1
UniProt Secondary Accession:P36406,Q9BZY4, Q9BZY5,
UniProt Related Accession:P36406
Molecular Weight:61,068 Da
NCBI Full Name:E3 ubiquitin-protein ligase TRIM23
NCBI Synonym Full Names:tripartite motif containing 23
NCBI Official Symbol:TRIM23  
NCBI Official Synonym Symbols:ARD1; ARFD1; RNF46  
NCBI Protein Information:E3 ubiquitin-protein ligase TRIM23
UniProt Protein Name:E3 ubiquitin-protein ligase TRIM23
UniProt Synonym Protein Names:ADP-ribosylation factor domain-containing protein 1; GTP-binding protein ARD-1; RING finger protein 46; Tripartite motif-containing protein 23
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:TRIM23  
UniProt Entry Name:TRI23_HUMAN
Anti-TRIM23 Polyclonal Antibody (CAB8329)Western blot analysis of extracts of various cell lines, using TRIM23 antibody (CAB8329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-TRIM23 Polyclonal Antibody (CAB8329)Immunofluorescence analysis of NIH/3T3 cells using TRIM23 Rabbit pAb (CAB8329) at dilution of 1:100. Blue: DAPI for nuclear staining.
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