Name: Anti-TRIM11 Antibody (CAB13887)
Brand: Immunology Antibodies 1
SKU: CAB13887
Availability: OutOfStock

Antibody Name:	Anti-TRIM11 Antibody
Antibody SKU:	CAB13887
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-468 of human TRIM11 (NP_660215.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, 293T, A-431

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-468 of human TRIM11 (NP_660215.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELR TVCR VPGL VETL RRFR GDVT LDPD TANP ELIL SEDR RSVQ RGDL RQAL PDSP ERFD PGPC VLGQ ERFT SGRH YWEV EVGD RTSW ALGV CREN VNRK EKGE LSAG NGFW ILVF LGSY YNSS ERAL APLR DPPR RVGI FLDY EAGH LSFY SATD GSLL FIFP EIPF SGTL RPLF SPLS SSPT PMTI CRPK GGSG DTLA PQ
Gene ID:	81559
Uniprot:	Q96F44
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	42kDa/52kDa
Observed MW:	52kDa

Synonyms:	TRIM11, BIA1, RNF92
Background:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified.

UniProt Protein Function:	TRIM11: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase Chromosomal Location of Human Ortholog: 1q42.13 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: innate immune response; negative regulation of viral transcription; negative regulation of virion penetration into host cell; positive regulation of virion penetration into host cell
NCBI Summary:	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96F44
NCBI GenInfo Identifier:	26400672
NCBI Gene ID:	81559
NCBI Accession:	Q96F44.2
UniProt Secondary Accession:	Q96F44,Q5VSU1, Q8NCA6, Q9C022, A6NKE2, B2RB82, B3KUS3 B4DX88,
UniProt Related Accession:	Q96F44
Molecular Weight:	52,646 Da
NCBI Full Name:	E3 ubiquitin-protein ligase TRIM11
NCBI Synonym Full Names:	tripartite motif containing 11
NCBI Official Symbol:	TRIM11
NCBI Official Synonym Symbols:	BIA1; RNF92
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM11
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM11
UniProt Synonym Protein Names:	Protein BIA1; RING finger protein 92; Tripartite motif-containing protein 11
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	TRIM11
UniProt Entry Name:	TRI11_HUMAN

Western blot analysis of extracts of various cell lines, using TRIM11 antibody (CAB13887) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

TRIM11 Antibody (PACO12902)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)