Cell Biology Antibodies 2

Anti-TRIB2 Antibody (CAB11661)

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SKU:
CAB11661
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TRIB2 Antibody
Antibody SKU:CAB11661
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human TRIB2 (NP_067675.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:K-562, 293T, Raji, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human TRIB2 (NP_067675.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNIH RSTP ITIA RYGR SRNK TQDF EELS SIRS AEPS QSFS PNLG SPSP PETP NLSH CVSC IGKY LLLE PLEG DHVF RAVH LHSG EELV CKVF DISC YQES LAPC FCLS AHSN INQI TEII
Gene ID:28951
Uniprot:Q92519
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:38kDa
Observed MW:49kDa
Synonyms:TRIB2, C5FW, GS3955, TRB2
Background:This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:TRB2: Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
UniProt Protein Details:

Protein type:CAMK group; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Trbl family

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: cytoplasm; nucleus

Molecular Function:mitogen-activated protein kinase kinase binding; nucleotide binding; protein kinase activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: negative regulation of fat cell differentiation; negative regulation of interleukin-10 biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity

NCBI Summary:This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:Q92519
NCBI GenInfo Identifier:74762638
NCBI Gene ID:28951
NCBI Accession:Q92519.1
UniProt Secondary Accession:Q92519,B2R851, D6W510,
UniProt Related Accession:Q92519
Molecular Weight:39kDa
NCBI Full Name:Tribbles homolog 2
NCBI Synonym Full Names:tribbles pseudokinase 2
NCBI Official Symbol:TRIB2  
NCBI Official Synonym Symbols:C5FW; TRB2; GS3955  
NCBI Protein Information:tribbles homolog 2
UniProt Protein Name:Tribbles homolog 2
Protein Family:Tribbles
UniProt Gene Name:TRIB2  
Anti-TRIB2 Antibody (CAB11661)Western blot analysis of extracts of various cell lines, using TRIB2 antibody (CAB11661) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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system_update_altDatasheetHuman TRIB2/TRB2 Recombinant Protein Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, T