Cell Biology Antibodies 5
Anti-TRHR Antibody (CAB15235)
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- SKU:
- CAB15235
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TRHR Antibody |
| Antibody SKU: | CAB15235 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-398 of human TRHR (NP_003292.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-398 of human TRHR (NP_003292.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YNLM SQKF RAAF RKLC NCKQ KPTE KPAN YSVA LNYS VIKE SDHF STEL DDIT VTDT YLSA TKVS FDDT CLAS EVSF SQS |
| Gene ID: | 7201 |
| Uniprot: | P34981 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 45kDa |
| Observed MW: | 55kDa |
| Synonyms: | TRHR, TRH-R |
| Background: | This gene encodes a G protein-coupled receptor for thyrotropin-releasing hormone (TRH). Upon binding to TRH, this receptor activates the inositol phospholipid-calcium-protein kinase C transduction pathway. Mutations in this gene have been associated with generalized thyrotropin-releasing hormone resistance. |
| UniProt Protein Function: | TRHR: Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 8q23 Cellular Component: integral to plasma membrane; plasma membrane |
| NCBI Summary: | This gene encodes a G protein-coupled receptor for thyrotropin-releasing hormone (TRH). Upon binding to TRH, this receptor activates the inositol phospholipid-calcium-protein kinase C transduction pathway. Mutations in this gene have been associated with generalized thyrotropin-releasing hormone resistance. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P34981 |
| NCBI GenInfo Identifier: | 464921 |
| NCBI Gene ID: | 7201 |
| NCBI Accession: | P34981.1 |
| UniProt Secondary Accession: | P34981,Q2M339, |
| UniProt Related Accession: | P34981 |
| Molecular Weight: | 45,085 Da |
| NCBI Full Name: | Thyrotropin-releasing hormone receptor |
| NCBI Synonym Full Names: | thyrotropin releasing hormone receptor |
| NCBI Official Symbol: | TRHR |
| NCBI Official Synonym Symbols: | TRH-R |
| NCBI Protein Information: | thyrotropin-releasing hormone receptor |
| UniProt Protein Name: | Thyrotropin-releasing hormone receptor |
| UniProt Synonym Protein Names: | Thyroliberin receptor |
| Protein Family: | Thyrotropin-releasing hormone receptor |
| UniProt Gene Name: | TRHR |
| UniProt Entry Name: | TRFR_HUMAN |
 | Western blot analysis of extracts of U-87MG cells, using TRHR antibody (CAB15235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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