Antibody Name:	Anti-TRH Antibody
Antibody SKU:	CAB14472
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRH (NP_009048.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, BxPC-3, A-549, DU145, Mouse brain, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRH (NP_009048.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEEE EGGA VGPH KRQH PGRR EDEA SWSV DVTQ HKRQ HPGR RSPW LAYA VPKR QHPG RRLA DPKA QRSW EEEE EEEE REED LMPE KRQH PGKR ALGG PCGP Q
Gene ID:	7200
Uniprot:	P20396
Cellular Location:	Secreted
Calculated MW:	27kDa
Observed MW:	27kDa

Synonyms:

TRH, Pro-TRH, TRF

Background:

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism.

UniProt Protein Function:	TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3q13.3-q21 Cellular Component: extracellular region; nucleus; plasma membrane; secretory granule Biological Process: cell-cell signaling; eating behavior; histamine metabolic process; negative regulation of glutamate secretion; positive regulation of gamma-aminobutyric acid secretion; positive regulation of insulin secretion; signal transduction Disease: Thyrotropin-releasing Hormone Deficiency
NCBI Summary:	This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]
UniProt Code:	P20396
NCBI GenInfo Identifier:	135841
NCBI Gene ID:	7200
NCBI Accession:	P20396.1
UniProt Secondary Accession:	P20396,Q2TB83, B2R8R1,
UniProt Related Accession:	P20396
Molecular Weight:	27,404 Da
NCBI Full Name:	Pro-thyrotropin-releasing hormone
NCBI Synonym Full Names:	thyrotropin releasing hormone
NCBI Official Symbol:	TRH
NCBI Official Synonym Symbols:	TRF; Pro-TRH
NCBI Protein Information:	thyrotropin releasing hormone
UniProt Protein Name:	Pro-thyrotropin-releasing hormone
UniProt Synonym Protein Names:	Prothyroliberin
Protein Family:	Thyrotropin-releasing hormone
UniProt Gene Name:	TRH
UniProt Entry Name:	TRH_HUMAN

	Western blot analysis of extracts of various cell lines, using TRH antibody (CAB14472) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Blue: DAPI for nuclear staining.