Antibody Name:	Anti-TREM2 Antibody
Antibody SKU:	CAB10482
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human TREM2 (NP_061838.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human TREM2 (NP_061838.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HNTT VFQG VAGQ SLQV SCPY DSMK HWGR RKAW CRQL GEKG PCQR VVST HNLW LLSF LRRW NGST AITD DTLG GTLT ITLR NLQP HDAG LYQC QSLH GSEA DTLR KVLV EVLA DPLD HRDA GDLW FPGE SESF EDAH VEHS IS
Gene ID:	54209
Uniprot:	Q9NZC2
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	24kDa/25kDa
Observed MW:	30-50KDa/28KDa

Synonyms:

TREM2, TREM-2, Trem2a, Trem2b, Trem2c

Background:

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: integral to membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:receptor activity Biological Process: humoral immune response; innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation; regulation of immune response Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy
NCBI Summary:	This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
UniProt Code:	Q9NZC2
NCBI GenInfo Identifier:	50401689
NCBI Gene ID:	54209
NCBI Accession:	Q9NZC2.1
UniProt Secondary Accession:	Q9NZC2,Q8N5H8, Q8WYN6,
UniProt Related Accession:	Q9NZC2
Molecular Weight:	25kDa
NCBI Full Name:	Triggering receptor expressed on myeloid cells 2
NCBI Synonym Full Names:	triggering receptor expressed on myeloid cells 2
NCBI Official Symbol:	TREM2
NCBI Official Synonym Symbols:	TREM-2; Trem2a; Trem2b; Trem2c
NCBI Protein Information:	triggering receptor expressed on myeloid cells 2
UniProt Protein Name:	Triggering receptor expressed on myeloid cells 2
UniProt Synonym Protein Names:	Triggering receptor expressed on monocytes 2
Protein Family:	Triggering receptor expressed on myeloid cells
UniProt Gene Name:	TREM2

	Western blot analysis of extracts of various cell lines, using TREM2 antibody (CAB10482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat spleen using TREM2 Rabbit pAb (CAB10482) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-251MG cells using TREM2 antibody (CAB10482) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse lung cells using TREM2 antibody (CAB10482) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Rat lung using TREM2 antibody (CAB10482) at dilution of 1:100. Blue: DAPI for nuclear staining.