Epigenetics & Nuclear Signaling Antibodies 1
Anti-TRDMT1 Antibody (CAB10535)
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- SKU:
- CAB10535
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TRDMT1 Antibody |
| Antibody SKU: | CAB10535 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 192-391 of human TRDMT1 (NP_004403.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SKOV3, 293T, DU145, HeLa, NCI-H460 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 192-391 of human TRDMT1 (NP_004403.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VHPQ KYAM DVEN KIQE KNVE PNIS FDGS IQCS GKDA ILFK LETA EEIH RKNQ QDSD LSVK MLKD FLED DTDV NQYL LPPK SLLR YALL LDIV QPTC RRSV CFTK GYGS YIEG TGSV LQTA EDVQ VENI YKSL TNLS QEEQ ITKL LILK LRYF TPKE IANL LGFP PEFG FPEK ITVK QRYR LLGN SLNV HVVA KLIK ILYE |
| Gene ID: | 1787 |
| Uniprot: | O14717 |
| Cellular Location: | Nucleus |
| Calculated MW: | 6kDa/7kDa/12kDa/39kDa/41kDa/44kDa |
| Observed MW: | 45kDa |
| Synonyms: | TRDMT1, DMNT2, DNMT2, MHSAIIP, PUMET, RNMT1 |
| Background: | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. |
| UniProt Protein Function: | TRDMT1: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Belongs to the C5-methyltransferase family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.204; Methyltransferase; Methyltransferase, tRNA Chromosomal Location of Human Ortholog: 10p13 Cellular Component: cytoplasm; nucleoplasm Molecular Function:tRNA (cytosine-5-)-methyltransferase activity; tRNA methyltransferase activity Biological Process: tRNA methylation; tRNA modification |
| NCBI Summary: | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010] |
| UniProt Code: | O14717 |
| NCBI GenInfo Identifier: | 17374834 |
| NCBI Gene ID: | 1787 |
| NCBI Accession: | O14717.1 |
| UniProt Secondary Accession: | O14717,O43669, Q86WW6, B0YJ02, B0YJ03, B0YJ07, B0YJ08 |
| UniProt Related Accession: | O14717 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | tRNA (cytosine(38)-C(5))-methyltransferase |
| NCBI Synonym Full Names: | tRNA aspartic acid methyltransferase 1 |
| NCBI Official Symbol: | TRDMT1 |
| NCBI Official Synonym Symbols: | DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP |
| NCBI Protein Information: | tRNA (cytosine(38)-C(5))-methyltransferase |
| UniProt Protein Name: | tRNA (cytosine(38)-C(5))-methyltransferase |
| UniProt Synonym Protein Names: | DNA (cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; M.HsaIIP; PuMet |
| Protein Family: | tRNA |
| UniProt Gene Name: | TRDMT1 |
 | Western blot analysis of extracts of various cell lines, using TRDMT1 antibody (CAB10535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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