Description
Antibody Name: | Anti-TRAPPC2 Antibody (CAB20890) |
Antibody SKU: | CAB20890 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human TRAPPC2. |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | SKOV3, PC-3, SH-SY5Y, Mouse brain, Mouse thymus, Rat brain |
Immunogen: | Recombinant protein of Human TRAPPC2. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Nucleus, perinuclear region |
Calculated MW: | 14kDa/16kDa/20kDa |
Observed MW: | 14KDa |
Synonyms: | TRAPPC2, MIP2A, SEDL, SEDT, TRAPPC2P1, TRS20, ZNF547L, hYP38334 |
Background: | The protein encoded by this gene is thought to be part of a large multi-subunit complex involved in the targeting and fusion of endoplasmic reticulum-to-Golgi transport vesicles with their acceptor compartment. In addition, the encoded protein can bind c-myc promoter-binding protein 1 and block its transcriptional repression capability. Mutations in this gene are a cause of spondyloepiphyseal dysplasia tarda (SEDT). A processed pseudogene of this gene is located on chromosome 19, and other pseudogenes are found on chromosomes 8 and Y. Alternatively spliced transcript variants have been found for this gene. |
Immunohistochemistry of paraffin-embedded rat brain using TRAPPC2 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human small intestine using TRAPPC2 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse spleen using TRAPPC2 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using TRAPPC2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |