Immunology Antibodies 3

Anti-Transportin-1 Antibody (CAB9435)

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SKU:
CAB9435
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Transportin-1 Antibody
Antibody SKU:CAB9435
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human TNPO1 (NP_002261.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, U-87MG, DU145, HeLa, Mouse kidney, Rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human TNPO1 (NP_002261.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QFIR PWCT SLRN IRDN EEKD SAFR GICT MISV NPSG VIQD FIFF CDAV ASWI NPKD DLRD MFCK ILHG FKNQ VGDE NWRR FSDQ FPLP LKER LAAF YGV
Gene ID:3842
Uniprot:Q92973
Cellular Location:Cytoplasm, Nucleus
Calculated MW:96kDa/101kDa/102kDa
Observed MW:85-102kDa
Synonyms:TNPO1, IPO2, KPNB2, MIP, MIP1, TRN
Background:This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins.
UniProt Protein Function:KPNB2: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in nuclear import of M9- containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Belongs to the importin beta family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Karyopherin; RNA-binding; Nuclear import

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; cytosol; nuclear membrane; nucleus

Molecular Function:nuclear localization sequence binding; protein binding; protein transporter activity

Biological Process: NLS-bearing substrate import into nucleus; protein import into nucleus, docking; protein import into nucleus, translocation; regulation of mRNA stability; ribosomal protein import into nucleus

NCBI Summary:This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q92973
NCBI GenInfo Identifier:259016171
NCBI Gene ID:3842
NCBI Accession:Q92973.2
UniProt Secondary Accession:Q92973,Q92957, Q92975, B4DVC6,
UniProt Related Accession:Q92973
Molecular Weight:96,901 Da
NCBI Full Name:Transportin-1
NCBI Synonym Full Names:transportin 1
NCBI Official Symbol:TNPO1  
NCBI Official Synonym Symbols:MIP; TRN; IPO2; MIP1; KPNB2  
NCBI Protein Information:transportin-1
UniProt Protein Name:Transportin-1
UniProt Synonym Protein Names:Importin beta-2; Karyopherin beta-2; M9 region interaction protein; MIP
UniProt Gene Name:TNPO1  
UniProt Entry Name:TNPO1_HUMAN
Anti-Transportin-1 Polyclonal Antibody (CAB9435)Western blot analysis of extracts of various cell lines, using TNPO1 antibody (CAB9435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
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