Immunology Antibodies 1

Anti-TRAM1 Antibody (CAB15142)

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SKU:
CAB15142
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-TRAM1 Antibody
Antibody SKU:CAB15142
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 305-374 of human TRAM1 (NP_055109.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-251MG, mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 305-374 of human TRAM1 (NP_055109.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CVTQ AFMM WKFI NFQL RRWR EHSA FQAP AVKK KPTV TKGR SSKK GTEN GVNG TLTS NVAD SPRN KKEK SS
Gene ID:23471
Uniprot:Q15629
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:39kDa/43kDa
Observed MW:45kDa
Synonyms:TRAM1, PNAS8, TRAM, TRAMP
Background:This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause.
UniProt Protein Function:TRAM1: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Belongs to the TRAM family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Receptor, misc.; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function:protein binding; receptor activity

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; viral reproduction; gene expression; cotranslational protein targeting to membrane

NCBI Summary:This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
UniProt Code:Q15629
NCBI GenInfo Identifier:18202507
NCBI Gene ID:23471
NCBI Accession:Q15629.3
UniProt Related Accession:Q15629
Molecular Weight:
NCBI Full Name:Translocating chain-associated membrane protein 1
NCBI Synonym Full Names:translocation associated membrane protein 1
NCBI Official Symbol:TRAM1  
NCBI Official Synonym Symbols:TRAM; PNAS8; TRAMP  
NCBI Protein Information:translocating chain-associated membrane protein 1
UniProt Protein Name:Translocating chain-associated membrane protein 1
Protein Family:Translocating chain-associated membrane protein
UniProt Gene Name:TRAM1  
UniProt Entry Name:TRAM1_HUMAN
Anti-TRAM1 Antibody (CAB15142)Western blot analysis of extracts of various cell lines, using TRAM1 antibody (CAB15142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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