Cell Death Antibodies 1

Anti-TRAF5 Antibody (CAB2536)

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SKU:
CAB2536
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-TRAF5 Antibody
Antibody SKU:CAB2536
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human TRAF5 (NP_665702.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:OVCAR3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human TRAF5 (NP_665702.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAYS EEHK GMPC GFIR QNSG NSIS LDFE PSIE YQFV ERLE ERYK CAFC HSVL HNPH QTGC GHRF CQHC ILSL RELN TVPI
Gene ID:7188
Uniprot:O00463
Cellular Location:Cytoplasm, cytosol
Calculated MW:52kDa/64kDa/65kDa
Observed MW:64kDa
Synonyms:TRAF5, MGC:39780, RNF84
Background:The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TRAF5: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Belongs to the TNF receptor-associated factor family. A subfamily.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: centrosome; internal side of plasma membrane; cytoplasm; cytosol

Molecular Function:protein binding; signal transducer activity; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; positive regulation of cell proliferation; protein ubiquitination; positive regulation of transcription factor activity; signal transduction; activation of NF-kappaB transcription factor

NCBI Summary:The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:O00463
NCBI GenInfo Identifier:30580600
NCBI Gene ID:7188
NCBI Accession:O00463.2
UniProt Related Accession:O00463
Molecular Weight:Observed: 55 kDaPredicted: 65 kDa
NCBI Full Name:TNF receptor-associated factor 5
NCBI Synonym Full Names:TNF receptor associated factor 5
NCBI Official Symbol:TRAF5  
NCBI Official Synonym Symbols:RNF84; MGC:39780  
NCBI Protein Information:TNF receptor-associated factor 5
UniProt Protein Name:TNF receptor-associated factor 5
UniProt Synonym Protein Names:RING finger protein 84
Protein Family:TNF receptor-associated factor
UniProt Gene Name:TRAF5  
UniProt Entry Name:TRAF5_HUMAN
Anti-TRAF5 Antibody (CAB2536)Western blot analysis of extracts of OVCAR3 cells, using TRAF5 antibody (CAB2536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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