Antibody Name:	Anti-TRAF3 Antibody
Antibody SKU:	CAB3094
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human TRAF3

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, SW480, MCF7, U-937, ES-2, Raji, Mouse gastrocnemius muscle, Mouse spleen

Immunogen:	A synthetic peptide of human TRAF3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7187
Uniprot:	Q13114
Cellular Location:	Cytoplasm, Endosome, Mitochondrion
Calculated MW:	55kDa/64kDa
Observed MW:	70kDa

Synonyms:

TRAF3, CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1

Background:

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported.

UniProt Protein Function:	TRAF3: Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. Homotrimer. Heterotrimer with TRAF2 and TRAF5. Interacts with LTBR/TNFRSF3, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF13C TNFRSF17/BCMA, TLR4 and EDAR. Interacts with MAP3K5, MAP3K14, TRAIP/TRIP, TDP2/TTRAP, TANK/ITRAF and TRAF3IP1. Interaction with TNFRSF5/CD40 is modulated by TANK/ITRAF, which competes for the same binding site. Interacts with TICAM1. Interacts with TRAFD1. Interacts with OTUB1, OTUB2 and OTUD5. Interacts with RNF216, MAVS, OPTN and TBK1. Identified in a complex with TRAF2, MAP3K14 and BIRC3. Interacts with BIRC2 and BIRC3. Upon exposure to bacterial lipopolysaccharide (LPS), recruited to a transient complex containing TLR4, TRAF3, TRAF6, IKBKG, MAP3K7, MYD88, TICAM1, BIRC2, BIRC3 and UBE2N. Interacts with Epstein-Barr virus protein LMP1. Interacts (via RING-type zinc finger domain) with SRC. Belongs to the TNF receptor-associated factor family. A subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 14q32.32 Cellular Component: internal side of plasma membrane; mitochondrion; cytosol; endosome Molecular Function:protein binding; signal transducer activity; zinc ion binding; ubiquitin protein ligase binding; thioesterase binding; ubiquitin-protein ligase activity; tumor necrosis factor receptor binding; protein kinase binding; ligase activity Biological Process: apoptosis; MyD88-independent toll-like receptor signaling pathway; regulation of defense response to virus; protein ubiquitination; toll-like receptor 3 signaling pathway; signal transduction; Toll signaling pathway; regulation of cytokine production; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; inhibition of NF-kappaB transcription factor; regulation of proteolysis; toll-like receptor signaling pathway; innate immune response; regulation of interferon-beta production; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway Disease: Herpes Simplex Encephalitis, Susceptibility To, 3
NCBI Summary:	The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010]
UniProt Code:	Q13114
NCBI GenInfo Identifier:	116242824
NCBI Gene ID:	7187
NCBI Accession:	Q13114.2
UniProt Secondary Accession:	Q13114,Q12990, Q13076, Q13947, Q6AZX1, Q9UNL1, B7Z8C4
UniProt Related Accession:	Q13114
Molecular Weight:	55,306 Da
NCBI Full Name:	TNF receptor-associated factor 3
NCBI Synonym Full Names:	TNF receptor-associated factor 3
NCBI Official Symbol:	TRAF3
NCBI Official Synonym Symbols:	CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
NCBI Protein Information:	TNF receptor-associated factor 3; CD40 binding protein; CD40 associated protein 1; LMP1-associated protein 1; CD40 receptor associated factor 1
UniProt Protein Name:	TNF receptor-associated factor 3
UniProt Synonym Protein Names:	CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1
Protein Family:	TNF receptor-associated factor
UniProt Gene Name:	TRAF3
UniProt Entry Name:	TRAF3_HUMAN

	Western blot analysis of extracts of various cell lines, using TRAF3 antibody (CAB3094) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using TRAF3 antibody (CAB3094). Blue: DAPI for nuclear staining.