Cell Biology Antibodies 9

Anti-TPT1 Antibody (CAB5442)

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SKU:
CAB5442
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TPT1 Antibody
Antibody SKU:CAB5442
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TPT1 (NP_003286.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, MCF7, K-562, THP-1, U-251MG, 293T, HepG2, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TPT1 (NP_003286.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIIY RDLI SHDE MFSD IYKI REIA DGLC LEVE GKMV SRTE GNID DSLI GGNA SAEG PEGE GTES TVIT GVDI VMNH HLQE TSFT KEAY KKYI KDYM KSIK GKLE EQRP ERVK PFMT GAAE QIKH ILAN FKNY QFFI GENM NPDG MVAL LDYR EDGV TPYM IFFK DGLE MEKC
Gene ID:7178
Uniprot:P13693
Cellular Location:Cytoplasm
Calculated MW:15kDa/19kDa
Observed MW:22kDa
Synonyms:TPT1, HRF, TCTP, p02, p23
Background:
UniProt Protein Function:TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.
UniProt Protein Details:

Protein type:Calcium-binding; Microtubule-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus

Molecular Function:protein binding; calcium ion binding

Biological Process: regulation of apoptosis; cellular calcium ion homeostasis; response to virus; calcium ion transport; negative regulation of apoptosis

UniProt Code:P13693
NCBI GenInfo Identifier:136479
NCBI Gene ID:7178
NCBI Accession:P13693.1
UniProt Secondary Accession:P13693,Q6YLS2, Q7Z4J4, Q8TBK7, Q96EE2, Q9UC70, B2R7E5
UniProt Related Accession:P13693
Molecular Weight:172
NCBI Full Name:Translationally-controlled tumor protein
NCBI Synonym Full Names:tumor protein, translationally-controlled 1
NCBI Official Symbol:TPT1  
NCBI Official Synonym Symbols:HRF; p02; p23; TCTP  
NCBI Protein Information:translationally-controlled tumor protein; fortilin; histamine-releasing factor
UniProt Protein Name:Translationally-controlled tumor protein
UniProt Synonym Protein Names:Fortilin; Histamine-releasing factor; HRF; p23
Protein Family:TPT1-like protein
UniProt Gene Name:TPT1  
UniProt Entry Name:TCTP_HUMAN
Anti-TPT1 Antibody (CAB5442)Western blot analysis of extracts of various cell lines, using TPT1 antibody (CAB5442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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