Name: Anti-TPSB2 Antibody (CAB15887)
Brand: Immunology Antibodies 2
SKU: CAB15887
Availability: OutOfStock

Antibody Name:	Anti-TPSB2 Antibody
Antibody SKU:	CAB15887
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human TPSB2 (NP_077078.5).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	H-460, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human TPSB2 (NP_077078.5).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLNL LLLA LPVL ASRA YAAP APGQ ALQR VGIV GGQE APRS KWPW QVSL RVRD RYWM HFCG GSLI HPQW VLTA AHCV GPDV KDLA ALRV QLRE QHLY YQDQ LLPV SRII VHPQ FYTA QIGA DIAL LELE EPVN VSSH VHTV TLPP ASET FPPG MPCW VTGW GDVD NDER LPPP FPLK QVKV PIME NHIC DAKY HLGA YTGD DVRI VRDD MLCA GNTR RDSC QGDS GGPL VCKV NGTW LQAG VVSW GEGC AQPN RPGI YTRV TYYL DWIH HYVP KKP
Gene ID:	64499
Uniprot:	P20231
Cellular Location:	Secreted
Calculated MW:	30kDa
Observed MW:	30kDa

Synonyms:

TPSB2, TPS2, tryptaseB, tryptaseC

Background:

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

UniProt Protein Function:	TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.
UniProt Protein Details:	Protein type:EC 3.4.21.59; Secreted; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular region Molecular Function:protein binding; serine-type peptidase activity; serine-type endopeptidase activity Biological Process: proteolysis
NCBI Summary:	Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
UniProt Code:	P20231
NCBI GenInfo Identifier:	13775595
NCBI Gene ID:	7177
NCBI Accession:	NP_003285.2
UniProt Secondary Accession:	P20231,O95827, Q15664, Q9UQI6, Q9UQI7, D2E6S0, D2E6S2
UniProt Related Accession:	Q15661
Molecular Weight:	30,515 Da
NCBI Full Name:	tryptase alpha/beta-1
NCBI Synonym Full Names:	tryptase alpha/beta 1
NCBI Official Symbol:	TPSAB1
NCBI Official Synonym Symbols:	TPS1; TPS2; TPSB1
NCBI Protein Information:	tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
UniProt Protein Name:	Tryptase beta-2
UniProt Synonym Protein Names:	Tryptase II
Protein Family:	Tryptase
UniProt Gene Name:	TPSB2
UniProt Entry Name:	TRYB2_HUMAN

Western blot analysis of extracts of various cell lines, using TPSB2 antibody (CAB15887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human TPSB2 / Tryptase beta-2 ELISA Kit

Human Tryptase beta-2 (TPSB2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)